human ( Homo sapiens )
SQS

Western result: +

GST GST-SQS A0974 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: SQS
CBI Antibody Core Code: A0974
"Lot" Number: 1 Position: 100 (156-255)
% Identical Homology of Target to Mouse Protein: 86% (NP_034321.2| farnesyl diphosphate farnesyl transferase 1; squalene synthase)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
EFLDKHVTSEQEWDKYCHYVAGLVGIGLSRLFSASEFEDP
LVGEDTERANSMGLFLQKTNIIRDYLEDQQGGREFWPQEV
WSRYVKKLGDFAKPENIDLA

Antibody Name/Abbreviation: SQS-pep-Test1
CBI Antibody Core Code: A0975
"Lot" Number: 1 Position: 24 (344-367)
% Identical Homology of Target to Mouse Protein: 83% (NP_034321.2| farnesyl diphosphate farnesyl transferase 1; squalene synthase)
Molecular Weight of fusion protein: 29.64 KDa

Target sequence used to make this antibody:
EIYHRIPDSDPSSSKTRQIISTIR

Antibody Name/Abbreviation: SQS-test2
CBI Antibody Core Code: A0976
"Lot" Number: 1 Position: 60 (185-255)
% Identical Homology of Target to Mouse Protein: 85% (NP_034321.2| farnesyl diphosphate farnesyl transferase 1; squalene synthase)
Molecular Weight of fusion protein: 34.81 KDa

Target sequence used to make this antibody:
RLFSASEFEDPLVGEDTERANSMGLFLQKTNIIRDYLEDQ
QGGREFWPQEVWSRYVKKLGDFAKPENIDLA

Sequence Name: SQS
Full name: FDFT1
UniGene Number: N.A.
Accession Number: NP_004453
Source organism: human ( Homo sapiens )
Full length size (aa): 417
NCBI information page:

Full length sequence:
mefvkclghpeefynlvrfriggkrkvmpkmdqdslssslktcykylnqt
srsfaaviqaldgemrnavcifylvlraldtleddmtisvekkvpllhnf
hsflyqpdwrfmeskekdrqvledfptislefrnlaekyqtviadicrrm
gigmaefldkhvtseqewdkychyvaglvgiglsrlfsasefedplvged
teransmglflqktniirdyledqqggrefwpqevwsryvkklgdfakpe
nidlavqclnelitnalhhipdvitylsrlrnqsvfnfcaipqvmaiatl
aacynnqqvfkgavkirkgqavtlmmdatnmpavkaiiyqymeeiyhrip
dsdpsssktrqiistirtqnlpncqlisrshyspiylsfvmllaalswqy
latlsqvtedyvqtgeh