human ( Homo sapiens )
PASD1

Western result: +

GST GST-PASD1 A0977 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: PASD1
CBI Antibody Core Code: A0977
"Lot" Number: 1 Position: 100 (476-575)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
AFNQQQLVQQEQHLKEQQRQLREQLQQLREQRKVQKQKKM
QEKKKLQEQKMQEKKKLQEQRRQKKKKLQERKKWQGQMLQ
KEPEEEQQKQQLQEQPLKHN

Western result: +

control PASD1-pep-Test A0978 (fragment)

29.2 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: PASD1-pep-Test
CBI Antibody Core Code: A0978
"Lot" Number: 1 Position: 20 (531-550)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of GST-fusion: 29.2 KDa

Target sequence used to make this antibody:
KLQEQRRQKKKKLQERKKWQ

Sequence Name: PASD1
Full name: PASD1
UniGene Number: N.A.
Accession Number: NM_173493
Source organism: human ( Homo sapiens )
Full length size (aa): 773
NCBI information page:

Full length sequence:
MKMRGEKRRDKVNPKSSQRKLNWIPSFPTYDYFNQVTLQLLDGFMITLST
DGVIICVAENISSLLGHLPAEIVGKKLLSLLPDEEKDEVYQKIILKFPLL
NSETHIKFCCHLKRGNVEHGDSSAYENVKFIVNVRDICNEFPVVFSGLFS
SHLCADFAACVPQEDRLYLVGNVCILRTQLLQQLYTSKAVSDEAVLTQDS
DEEPFVGELSSSQGQRGHTSMKAVYVEPAAAAAAAAISDDQIDIAEVEQY
GPQENVHMFVDSDSTYCSSTVFLDTMPESPALSLQDFRGEPEVNPLYRAD
PVDLEFSVDQVDSVDQEGPMDQQDPENPVAPLDQAGLMDPVDPEDSVDLG
AAGASAQPLQPSSPVAYDIISQELELMKKLKEQLEERTWLLHDAIQNQQN
ALELMMDHLQKQPNTLRHVVIPDLQSSEAVPKKQQKQHAGQVKRPLPHPK
DVKCFCGLSLSNSLKNTGELQEPCVAFNQQQLVQQEQHLKEQQRQLREQL
QQLREQRKVQKQKKMQEKKKLQEQKMQEKKKLQEQRRQKKKKLQERKKWQ
GQMLQKEPEEEQQKQQLQEQPLKHNVIVGNERVQICLQNPRDVSVPLCNH
PVRFLQAQPIVPVQRAAEQQPSGFYQDENCGQQEDESQSFYPEAYQGPPV
NQLPLIDTSNSEAISSSSIPQFPITSDSTISTLETPQDYIRLWQELSDSL
GPVVQVNTWSCDEQGTLHGQPTYHQVQVSEVGVEGPPDPQAFQGPAAYQP
DQMRSAEQTRLMPAEQRDSNKPC