human ( Homo sapiens )
LRBA

Western result: +

GST GST-LRBA (WDL region) A1035a (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: LRBA (WDL region)
CBI Antibody Core Code: A1035a
"Lot" Number: 1 Position: 100 (2027-2126)
% Identical Homology of Target to Mouse Protein: 97% (NP_109620.1| LPS-responsive beige-like anchor)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
ATDEDILAKGKQSIRSQALGNQNSENEILLEGDDDTLSSV
DEKDLENLAGPVSLSTPAQLVAPSVVVKGTLSVTSSELYF
EVDEEDPNFKKIDPKILAYT

Antibody Name/Abbreviation: LRBA (WDL region)
CBI Antibody Core Code: A1035b
"Lot" Number: 1 Position: 20 (2035-2054)
% Identical Homology of Target to Mouse Protein: 94% (NP_109620.1| LPS-responsive beige-like anchor)
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
KGKQSIRSQALGNQNSENEI

Antibody Name/Abbreviation: LRBA
CBI Antibody Core Code: A1030b
"Lot" Number: 1 Position: 20 (1-20)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of GST-fusion: 32.2 KDa

Target sequence used to make this antibody:
MASEDNRVPSPPPTGDDGGG

Antibody Name/Abbreviation: LRBA
CBI Antibody Core Code: A1030a
"Lot" Number: 1 Position: 100 (1082-1181)
% Identical Homology of Target to Mouse Protein: 49% (NP_109620.1| LPS-responsive beige-like anchor)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
ISSPSEEDASEMPEFLDKSIVEEEEDDDYVELKVEGSPTE
EANLPTELQDNSLSPAASEAGEKLDMFGNDDKLIFQEGKP
VTEKQTDTETQDSKDSGIQT

Sequence Name: LRBA
Full name:
UniGene Number: N.A.
Accession Number: NM_006726
Source organism: human ( Homo sapiens )
Full length size (aa): 2863
NCBI information page:

Full length sequence:
MASEDNRVPSPPPTGDDGGGGGREETPTEGGALSLKPGLPIRGIRMKFAV
LTGLVEVGEVSNRDIVETVFNLLVGGQFDLEMNFIIQEGESINCMVDLLE
KCDITCQAEVWSMFTAILKKSIRNLQVCTEVGLVEKVLGKIEKVDNMIAD
LLVDMLGVLASYNLTVRELKLFFSKLQGDKGRWPPHAGKLLSVLKHMPQK
YGPDAFFNFPGKSAAAIALPPIAKWPYQNGFTFHTWLRMDPVNNINVDKD
KPYLYCFRTSKGLGYSAHFVGGCLIVTSIKSKGKGFQHCVKFDFKPQKWY
MVTIVHIYNRWKNSELRCYVNGELASYGEITWFVNTSDTFDKCFLGSSET
ADANRVFCGQMTAVYLFSEALNAAQIFAIYQLGLGYKGTFKFKAESDLFL
AEHHKLLLYDGKLSSAIAFTYNPRATDAQLCLESSPKDNPSIFVHSPHAL
MLQDVKAVLTHSIQSAMHSIGGVQVLFPLFAQLDYRQYLSDEIDLTICST
LLAFIMELLKNSIAMQEQMLACKGFLVIGYSLEKSSKSHVSRAVLELCLA
FSKYLSNLQNGMPLLKQLCDHVLLNPAIWIHTPAKVQLMLYTYLSTEFIG
TVNIYNTIRRVGTVLLIMHTLKYYYWAVNPQDRSGITPKGLDGPRPNQKE
MLSLRAFLLMFIKQLVMKDSGVKEDELQAILNYLLTMHEDDNLMDVLQLL
VALMSEHPNSMIPAFDQRNGLRVIYKLLASKSEGIRVQALKAMGYFLKHR
PPKRKAEVMLGHGLFSLLAERLMLQTNLITMTTYNVLFEILIEQIGTQVI
HKQHPDPDSSVKIQNPQILKVIATLLRNSPQCPESMEVRRAFLSDMIKLF
NNSRENRRSLLQCSVWQEWMLSLCYFNPKNSDEQKITEMVYAIFRILLYH
AVKYEWGGWRVWVDTLSITHSKVTFEIHKENLANIFREQQGKVDEEIGLC
SSTSVQAASGIRRDINVSVGSQQPDTKDSPVCPHFTTNGNENSSIEKTSS
LESASNIELQTTNTSYEEMKAEQENQELPDEGTLEETLTNETRNADDLEV
SSDIIEAVAISSNSFITTGKDSMTVSEVTASISSPSEEDASEMPEFLDKS
IVEEEEDDDYVELKVEGSPTEEANLPTELQDNSLSPAASEAGEKLDMFGN
DDKLIFQEGKPVTEKQTDTETQDSKDSGIQTMTASGSSAMSPETTVSQIA
VESDLGQMLEEGKKATNLTRETKLINDCHGSVSEASSEQKIAKLDVSNVA
TDTERLELKASPNVEAPQPHRHVLEISRQHEQPGQGIAPDAVNGQRRDSR
STVFRIPEFNWSQMHQRLLTDLLFSIETDIQMWRSHSTKTVMDFVNSSDN
VIFVHNTIHLISQVMDNMVMACGGILPLLSAATSATHELENIEPTQGLSI
EASVTFLQRLISLVDVLIFASSLGFTEIEAEKSMSSGGILRQCLRLVCAV
AVRNCLECQQHSQLKTRGDKALKPMHSLIPLGKSAAKSPVDIVTGGISPV
RDLDRLLQDMDINRLRAVVFRDIEDSKQAQFLALAVVYFISVLMVSKYRD
ILEPQNERHSQSCTETGSENENVSLSEITPAAFSTLTTASVEESESTSSA
RRRDSGIGEETATGLGSHVEVTPHTAPPGVSAGPDAISEVLSTLSLEVNK
SPETKNDRGNDLDTKATPSVSVSKNVNVKDILRSLVNIPADGVTVDPALL
PPACLGALGDLSVEQPVQFRSFDRSVIVAAKKSAVSPSTFNTSIPTNAVS
VVSSVDSAQASDMGGESPGSRSSNAKLPSVPTVDSVSQDPVSNMSITERL
EHALEKAAPLLREIFVDFAPFLSRTLLGSHGQELLIEGTSLVCMKSSSSV
VELVMLLCSQEWQNSIQKNAGLAFIELVNEGRLLSQTMKDHLVRVANEAE
FILSRQRAEDIHRHAEFESLCAQYSADKREDEKMCDHLIRAAKYRDHVTA
TQLIQKIINILTDKHGAWGNSAVSRPLEFWRLDYWEDDLRRRRRFVRNPL
GSTHPEATLKTAVEHVCIFKLRENSKATDEDILAKGKQSIRSQALGNQNS
ENEILLEGDDDTLSSVDEKDLENLAGPVSLSTPAQLVAPSVVVKGTLSVT
SSELYFEVDEEDPNFKKIDPKILAYTEGLHGKWLFTEIRSIFSRRYLLQN
TALEIFMANRVAVMFNFPDPATVKKVVNFLPRVGVGTSFGLPQTRRISLA
SPRQLFKASNMTQRWQHREISNFEYLMFLNTIAGRSYNDLNQYPVFPWVI
TNYESEELDLTLPTNFRDLSKPIGALNPKRAAFFAERYESWEDDQVPKFH
YGTHYSTASFVLAWLLRIEPFTTYFLNLQGGKFDHADRTFSSISRAWRNS
QRDTSDIKELIPEFYYLPEMFVNFNNYNLGVMDDGTVVSDVELPPWAKTS
EEFVHINRLALESEFVSCQLHQWIDLIFGYKQQGPEAVRALNVFYYLTYE
GAVNLNSITDPVLREAVEAQIRSFGQTPSQLLIEPHPPRGSAMQVSPLMF
TDKAQQDVIMVLKFPSNSPVTHVAANTQPGLATPAVITVTANRLFAVNKW
HNLPAHQGAVQDQPYQLPVEIDPLIASNTGMHRRQITDLLDQSIQVHSQC
FVITSDNRYILVCGFWDKSFRVYSTDTGRLIQVVFGHWDVVTCLARSESY
IGGNCYILSGSRDATLLLWYWNGKCSGIGDNPGSETAAPRAILTGHDYEV
TCAAVCAELGLVLSGSQEGPCLIHSMNGDLLRTLEGPENCLKPKLIQASR
EGHCVIFYENGLFCTFSVNGKLQATMETDDNIRAIQLSRDGQYLLTGGDR
GVVVVRQVSDLKQLFAYPGCDAGIRAMALSYDQRCIISGMASGSIVLFYN
DFNRWHHEYQTRY