human ( Homo sapiens )
Remmert human gene

Western result: +

GST GST-hCA A1034a (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: hCA
CBI Antibody Core Code: A1034a
"Lot" Number: 1 Position: 100 (1215-1314)
% Identical Homology of Target to Mouse Protein: 64% (XP_488514.1| hypothetical protein XP_488514)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
QSPKPSLAARPVIPQKPRTASRPDDIPDSPSSPKVALLPP
VLKKVPSDKERDGQSSPQPSPRTFSQEVSRRSWGQQAQEY
QEQKQRSSSKDGHQGSKSND

Antibody Name/Abbreviation: hCA
CBI Antibody Core Code: A1034b
"Lot" Number: 1 Position: 20 (1259-1278)
% Identical Homology of Target to Mouse Protein: 75% (XP_488514.1| hypothetical protein XP_488514)
Molecular Weight of GST-fusion: 32.2 KDa

Target sequence used to make this antibody:
VPSDKERDGQSSPQPSPRTF

Sequence Name: Remmert human gene
Full name: human CARMIL
UniGene Number: N.A.
Accession Number: BAC86250
Source organism: human ( Homo sapiens )
Full length size (aa): 1326
NCBI information page:

Full length sequence:
MTEESSDVPRELIESIKDVIGRKIKISVKKKVKLEVKGDKVENKVLVLTS
CRAFLVTARIPTKLELTFSYLEIHGVVCSKSAQMIVETEKCSISMKMASP
EDVSEVLAHIGTCLRKIFPGLSPVRIMKKVSMEPSERLASLQALWDSQTV
AEQGPCGGFSQMYACVCDWLGFSYREEVQWDVDTIYLTQDTRELNLQDFS
HLDHRDLIPIIAALEYNQWFTKLSSKDLKLSTDVCEQILRVVSRSNRLEE
LVLENAGLRTDFAQKLASALAHNPNSGLHTINLAGNPLEDRGVSSLSIQF
AKLPKGLKHLNLSKTSLSPKGVNSLSQSLSANPLTASTLVHLDLSGNVLR
GDDLSHMYNFLAQPNAIVHLDLSNTECSLDMVCGALLRGCLQYLAVLNLS
RTVFSHRKGKEVPPSFKQFFSSSLALMHINLSGTKLSPEPLKALLLGLAC
NHNLKGVSLDLSNCELRSGGAQVLEGCIAEIHNITSLDISDNGLESDLST
LIVWLSKNRSIQHLALGKNFNNMKSKNLTPVLDNLVQMIQDEESPLQSLS
LADSKLKTEVTIIINALGSNTSLTKVDISGNGMGDMGAKMLAKALQINTK
LRTVIWDKNNITAQGFQDIAVAMEKNYTLRFMPIPMYDASQALKTNPEKT
EDALQKIENYLLRNHETRKYLQEQAYRLQQGIVTSTTQQMIDRICVKVQD
HLNSLRNCGGDAIQEDLKSAERLMRDAKNSKTLLPNLYHVGGASWAGASG
LLSSPIQETLESMAGEVTRVVDEQLKALLESMVDAAENLCPNVMKKAHIR
QDLIHASTEKISIPRTFVKNVLLEQSGIDILNKISEVKLTVASFLSDRIV
DEILDALSHCHHKLADHFSRRGKTLPQQESLEIELAEEKPVKRSIITVEE
LTEIERLEDLDTCMMTPKSKRKSIHSRMLRPVSRAFEMEFDLDKALEEVP
IHIEDPPFPSLRQEKRSSGFISELPSEEGKKLEHFTKLRPKRNKKQQPTQ
AAVCAANIVSQDGEQNGLMGRVDEGVDEFFTKKVTKMDSKKWSTRGSESH
ELNEGGDEKKKRDSRKSSGFLNLIKSRSKSERPPTILMTEEPSSPKGAVR
SPPVDCPRKDTKAAEHNGNSERIEEIKTPDSFEESQGEEIGKVERSDSKS
SPQAGRRYGVQVMGSGLLAEMKAKQENRFGLGTPEKNTKAEPKAEAGSRS
RSSSSTPTSPKPLLQSPKPSLAARPVIPQKPRTASRPDDIPDSPSSPKVA
LLPPVLKKVPSDKERDGQSSPQPSPRTFSQEVSRRSWGQQAQEYQEQKQR
SSSKDGHQGSKSNDSGEEAEKEFIFV