human ( Homo sapiens )
Gbeta2-like

Western result: +

GST GST-Gbeta2-like (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Gbeta2-like
CBI Antibody Core Code: A0283
"Lot" Number: 1 Position: 100(201-300)
% Identical Homology of Target to Mouse Protein: 100% (NP_032169.1| guanine nucleotide binding protein, beta 2, related sequence 1; guanine nucleotide binding protein, beta-2, related sequence 1; guanine nucleotide binding protein related gene; guanine nucleotide binding protein (G protein), beta polypeptide)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
SPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALC
FSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSK
AEPPQCTSLAWSADGQTLFA

Sequence Name: Gbeta2-like
Full name: G protein beta peptide 2-like
UniGene Number: N.A.
Accession Number: NP_006089.1
Source organism: human ( Homo sapiens )
Full length size (aa): 317
NCBI information page: click here

Full length sequence:
MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDET
NYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRR
FVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEW
VSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTV
SPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCA
ATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFA
GYTDNLVRVWQVTIGTR