human ( Homo sapiens )
GDIB

Antibody Name/Abbreviation: GDIB-N
CBI Antibody Core Code: A0278
"Lot" Number: 1 Position: 100(1-100)
% Identical Homology of Target to Mouse Protein: 76% (NP_031512.1| Rho, GDP dissociation inhibitor (GDI) beta)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
MTEKAPEPHVEEDDDDELDSKLNYKPPPQKSLKELQEMDK
DDESLIKYKKTLLGDGPVVTDPKAPNVVVTRLTLVCESAP
GPITMDLTGDLEALKKETIV

Antibody Name/Abbreviation: GDIB-C
CBI Antibody Core Code: A0279
"Lot" Number: 2 Position: 100(102-201)
% Identical Homology of Target to Mouse Protein: 92% (NP_031512.1| Rho, GDP dissociation inhibitor (GDI) beta)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
KEGSEYRVKIHFKVNRDIVSGLKYVQHTYRTGVKVDKATF
MVGSYGPRPEEYEFLTPVEEAPKGMLARGTYHNKSFFTDD
DKQDHLSWEWNLSIKKEWTE

Other western:
Code: A0279
Lot: 1

Western result: +

GST GST-GDIB-C (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: GDIB
Full name: Rho GDP dissociation inhibitor (GDI) beta (ARHGDIB) (N-terminus)
UniGene Number: N.A.
Accession Number: NM_001175
Source organism: human ( Homo sapiens )
Full length size (aa): 201
NCBI information page: click here

Full length sequence:
MTEKAPEPHVEEDDDDELDSKLNYKPPPQKSLKELQEMDKDDESLIKYKK
TLLGDGPVVTDPKAPNVVVTRLTLVCESAPGPITMDLTGDLEALKKETIV
LKEGSEYRVKIHFKVNRDIVSGLKYVQHTYRTGVKVDKATFMVGSYGPRP
EEYEFLTPVEEAPKGMLARGTYHNKSFFTDDDKQDHLSWEWNLSIKKEWT
E