human ( Homo sapiens )
SULF2

Western result: +

GST GST-SULF2 A1078a (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: SULF2
CBI Antibody Core Code: A1078a
"Lot" Number: 1 Position: 100 (415-514)
% Identical Homology of Target to Mouse Protein: 85% (XP_358343.2| sulfatase 2)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
ERGKLLHKRDNDKVDAQEENFLPKYQRVKDLCQRAEYQTA
CEQLGQKWQCVEDATGKLKLHKCKGPMRLGGSRALSNLVP
KYYGQGSEACTCDSGDYKLS

Antibody Name/Abbreviation: SULF2
CBI Antibody Core Code: A1078b
"Lot" Number: 1 Position: 20 (563-582)
% Identical Homology of Target to Mouse Protein: 85% (XP_358343.2| sulfatase 2)
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
TKRHWPGAPEDQDDKDGGDF

Sequence Name: SULF2
Full name: Homo sapiens sulfatase 2
UniGene Number: N.A.
Accession Number: NM_018837
Source organism: human ( Homo sapiens )
Full length size (aa): 870
NCBI information page:

Full length sequence:
MGPPSLVLCLLSATVFSLLGGSSAFLSHHRLKGRFQRDRRNIRPNIILVL
TDDQDVELGSMQVMNKTRRIMEQGGAHFINAFVTTPMCCPSRSSILTGKY
VHNHNTYTNNENCSSPSWQAQHESRTFAVYLNSTGYRTAFFGKYLNEYNG
SYVPPGWKEWVGLLKNSRFYNYTLCRNGVKEKHGSDYSKDYLTDLITNDS
VSFFRTSKKMYPHRPVLMVISHAAPHGPEDSAPQYSRLFPNASQHITPSY
NYAPNPDKHWIMRYTGPMKPIHMEFTNMLQRKRLQTLMSVDDSMETIYNM
LVETGELDNTYIVYTADHGYHIGQFGLVKGKSMPYEFDIRVPFYVRGPNV
EAGCLNPHIVLNIDLAPTILDIAGLDIPADMDGKSILKLLDTERPVNRFH
LKKKMRVWRDSFLVERGKLLHKRDNDKVDAQEENFLPKYQRVKDLCQRAE
YQTACEQLGQKWQCVEDATGKLKLHKCKGPMRLGGSRALSNLVPKYYGQG
SEACTCDSGDYKLSLAGRRKKLFKKKYKASYVRSRSIRSVAIEVDGRVYH
VGLGDAAQPRNLTKRHWPGAPEDQDDKDGGDFSGTGGLPDYSAANPIKVT
HRCYILENDTVQCDLDLYKSLQAWKDHKLHIDHEIETLQNKIKNLREVRG
HLKKKRPEECDCHKISYHTQHKGRLKHRGSSLHPFRKGLQEKDKVWLLRE
QKRKKKLRKLLKRLQNNDTCSMPGLTCFTHDNQHWQTAPFWTLGPFCACT
SANNNTYWCMRTINETHNFLFCEFATGFLEYFDLNTDPYQLMNAVNTLDR
DVLNQLHVQLMELRSCKGYKQCNPRTRNMDLGLKDGGSYEQYRQFQRRKW
PEMKRPSSKSLGQLWEGWEG