human ( Homo sapiens )
GlxI

Western result: +

GST GST-GlxI (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: GlxI
CBI Antibody Core Code: A0277
"Lot" Number: 2 Position: 100(71-170)
% Identical Homology of Target to Mouse Protein: 95% (NP_079650.2| glyoxalase 1; glyoxalase 1 complex; glyoxalase 1 regulatory; glyoxalase 1 structural)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
FLAYEDKNDIPKEKDEKIAWALSRKATLELTHNWGTEDDE
TQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGVKFVK
KPDDGKMKGLAFIQDPDGYW

Sequence Name: GlxI
Full name: Human Glyoxalase I complex
UniGene Number: N.A.
Accession Number: 1QIPB
Source organism: human ( Homo sapiens )
Full length size (aa): 183
NCBI information page: click here

Full length sequence:
AEPQPPSGGLTDEAALSCCSDADPSTKDFLLQQTMLRVKDPKKSLDFYTR
VLGMTLIQKCDFPIMKFSLYFLAYEDKNDIPKEKDEKIAWALSRKATLEL
THNWGTEDDETQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGVKFVK
KPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM