human ( Homo sapiens )
GKT-AML8+3-1

Western result: +

GST GST-GKT-AML8+3-1 A1162a (fragment)

22.8 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: GKT-AML8+3-1
CBI Antibody Core Code: A1162a
"Lot" Number: 1 Position: 80 (1-80)
% Identical Homology of Target to Mouse Protein: 31% (XP_358348.2| potassium voltage-gated channel KQT-like protein 2)
Molecular Weight of fusion protein: 22.8 KDa

Target sequence used to make this antibody:
MFKRNDDKDSTSLGENTSDYSSQNAQHASASAFLQLILLV
QRVQTEGHFSSAMSAWAFYFCNFFPQEKTSMWKYKENITI

Western result: +

control GKT-AML8+3-1 A1162b (fragment)

32.2 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: GKT-AML8+3-1
CBI Antibody Core Code: A1162b
"Lot" Number: 1 Position: 20 (59-78)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of GST-fusion: 32.2 KDa

Target sequence used to make this antibody:
YFCNFFPQEKTSMWKYKENI

Sequence Name: GKT-AML8+3-1
Full name: GKT-AML8+3-1
UniGene Number: N.A.
Accession Number: AY371499
Source organism: human ( Homo sapiens )
Full length size (aa): 80
NCBI information page:

Full length sequence:
MFKRNDDKDSTSLGENTSDYSSQNAQHASASAFLQLILLVQRVQTEGHFS
SAMSAWAFYFCNFFPQEKTSMWKYKENITI