human ( Homo sapiens )
alpha-desmuslin

Western result: +

GST GST-A-SYN A1167a (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: A-SYN
CBI Antibody Core Code: A1167a
"Lot" Number: 1 Position: 100 (1312-1411)
% Identical Homology of Target to Mouse Protein: 50% (NP_964001.1| desmuslin isoform H)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
SIGPQRHQTTQQIVYHGLVPQLGESGDSESTVHGEGSADV
HQATHSHTSGRQTVMTEKSTFQSVVSESPQEDSAEDTSGA
EMTSGVSRSFRHIRLGPTET

Antibody Name/Abbreviation: A-SYN
CBI Antibody Core Code: A1167b
"Lot" Number: 1 Position: 20 (1309-1328)
% Identical Homology of Target to Mouse Protein: 35% (NP_964001.1| desmuslin isoform H)
Molecular Weight of GST-fusion: 32.2 KDa

Target sequence used to make this antibody:
RHISIGPQRHQTTQQIVYHG

Sequence Name: alpha-desmuslin
Full name:
UniGene Number: N.A.
Accession Number: NM0145728
Source organism: human ( Homo sapiens )
Full length size (aa): 1563
NCBI information page:

Full length sequence:
MLSLRLQTGPEKAELQELNARLYDYVCRVRELERENLLLEEELRGRRGRE
GLWAEGQARCAEEARSLRQQLDELSWATALAEGERDALRRELRELQRLDA
EERAARGRLDAELGAQQRELQEALGARAALEALLGRLQAERRGLDAAHER
DVRELRARAASLTMHFRARATGPAAPPPRLREVHDSYALLVAESWRETVQ
LYEDEVRELEEALRRGQESRLQAEEETRLCAQEAEALRREALGLEQLRAR
LEDALLRMREEYGIQAEERQRVIDCLEDEKATLTLAMADWLRDYQDLLQV
KTGLSLEVATYRALLEGESNPEIVIWAEHVENMPSEFRNKSYHYTDSLLQ
RENERNLFSRQKAPLASFNHSSALYSNLSGHRGSQTGTSIGGDARRGFLG
SGYSSSATTQQENSYGKAVSSQTNVRTFSPTYGLLRNTEAQVKTFPDRPK
AGDTREVPVYIGEDSTIARESYRDRRDKVAAGASESTRSNERTVILGKKT
EVKATREQERNRPETIRTKPEEKMFDSKEKASEERNLRWEELTKLDKEAR
QRESQQMKEKAKEKDSLKEKSVREREVPISLEVSQDRRAEVSPKGLQTPV
KDAGGGTGREAEARELRFRLGTSDATGSLQGDSMTETVAENIVTSILKQF
TQSPETEASADSFPDTKVTYVDRKELPGERKTKTEILVESKLTEDVDVSD
EAGLDYLLSKDIKEVGLKGKSAEQMIGDIINLGLKGREGRAKVVNVEIVE
EPVSYVSGEKPEEFSVPFKVEEVEDVSPGPWGLVKEEEGYGESDVTFSVN
QHRRTKQPQENTTHVEEVTEAGDSEGEQSYFVSTPDEHPGGHDRDDGSVY
GQIHIEEESTIRYSWQDEIVQGTRRRTQKDGAVGEKVVKPLDVPAPSLEG
DLGSTHWKEQARSGEFHAEPTVIEKEIKIPHEFHTSMKGISSKEPRQQLV
EVIGQLEETLPERMREELSALTREGQGGPGSVSVDVKKVQGAGGSSVTLV
AEVNVSQTVDADRLDLEEVSKDEASEMEKAVESVVRESLSRQRSPAPGSP
DEEGGAEAPAAGIRFRRWATRELYIPSGESEVAGGASHSSGQRTPQGPVS
ATVEVSSPTGFAQSQVLEDVSQAARHIKLGPSEVWRTERMSYEGPTAEVV
GVSAGGDLSQAASPTGASRSVRHVTLGPGQSPLSREVIFLGPAPACPEAW
GSPEPGPAESSADMDGSGRHSTFGCRQFHAEKEIIFQGPISAAGKVGDYF
ATEESVGTQTSVRQLQLGPKEGFSGQIQFTAPLSDKVELGVTGDSVHMEG
LPGSSTSIRHISIGPQRHQTTQQIVYHGLVPQLGESGDSESTVHGEGSAD
VHQATHSHTSGRQTVMTEKSTFQSVVSESPQEDSAEDTSGAEMTSGVSRS
FRHIRLGPTETETSEHIAIRGPVSRTFVLAGSADSPELGKLADSSRTLRH
IAPGPKETSFTFQMDVSNVEAIRSRTQEAGALGVSDRGSWRDADSRNDQA
VGVSFKASAGEGDQAHREQGKEQAMFDKKVQLQRMVDQRSVISDEKKVAL
LYLDNEEEENDGHWF