human ( Homo sapiens )
RNA Helicase A

Western result: +

GST GST-RNA Helicase A (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: RNA Helicase A
CBI Antibody Core Code: A1177
"Lot" Number: 1 Position: 100 (281-380)
% Identical Homology of Target to Mouse Protein: 67% (NP_031868.1| DEAH (Asp-Glu-Ala-His) box polypeptide 9; D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 9; DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 9; leukophysin; RNA helicase; nuclear DNA helicase II; DEAD/H (Asp-Glu-A)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
DLEHQLQNIIQELNLEILPPPEDPSVPVALNIGKLAQFEP
SQRQNQVGVVPWSPPQSNWNPWTSSNIDEGPLAFATPEQI
SMDLKNELMYQLEQDHDLQA

Sequence Name: RNA Helicase A
Full name:
UniGene Number: N.A.
Accession Number: Q08211
Source organism: human ( Homo sapiens )
Full length size (aa): 1270
NCBI information page:

Full length sequence:
mgdvknflyawcgkrkmtpsyeiravgnknrqkfmcevqvegynytgmgn
stnkkdaqsnaardfvnylvrineikseevpafgvaspppltdtpdttan
aegdlpttmggplpphlalkaennsevgasgygvpgptwdrganlkdyys
rkeeqevqatleseevdlnaglhgnwtlenakarlnqyfqkekiqgeyky
tqvgpdhnrsfiaemtiyikqlgrrifarehgsnkklaaqscalslvrql
yhlgvveaysgltkkkegetvepykvnlsqdlehqlqniiqelnleilpp
pedpsvpvalnigklaqfepsqrqnqvgvvpwsppqsnwnpwtssnideg
plafatpeqismdlknelmyqleqdhdlqailqerellpvkkfeseilea
isqnsvviirgatgcgkttqvpqfilddfiqndraaecnivvtqprrisa
vsvaervafergeepgkscgysvrfesilprphasimfctvgvllrklea
girgishvivdeiherdintdfllvvlrdvvqaypevrivlmsatidtsm
fceyffncpiievygrtypvqeyfledciqmthfvpppkdkkkkdkdddg
gedddancnlicgdeygpetrlsmsqlneketpfelieallkyietlnvp
gavlvflpgwnliytmqkhlemnphfgshryqilplhsqipreeqrkvfd
pvpvgvtkvilstniaetsitindvvyvidsckqkvklftahnnmtnyst
vwasktnleqrkgragrstagfcfhlcsrarferlethmtpemfrtplhe
ialsikllrlggigqflakaiepppldavieaehtlreldaldandeltp
lgrilaklpieprfgkmmimgcifyvgdaictiaaatcfpepfinegkrl
gyihrnfagnrfsdhvallsvfqawddarmggeeaeirfcehkrlnmatl
rmtweakvqlkeilinsgfpedclltqvftntgpdnnldvvisllafgvy
pnvcyhkekrkilttegrnalihkssvncpfssqdmkypspffvfgekir
traisakgmtlvpplqlllfaskkvqsdgqivlvddwiklqisheaaaci
tglraamealvvevtkqpaiisqldpvnermlnmirqisrpsaaginlmi
gstrygdgprppkmarydngsgyrrggssysgggygggyssggygsggyg
gsansfragygagvgggyrgvsrggfrgnsggdyrgpsggyrgsggfqrg
ggrgaygtgyfgqgrggggy