human ( Homo sapiens )
hApoAV-TM2

Western result:

control hApoAV-TM2 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: hApoAV-TM2
CBI Antibody Core Code: 1Ab109
"Lot" Number: 1 Position: To be posted
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
EKLRPLSGSEAPRLPQDPVG

Sequence Name: hApoAV-TM2
Full name: apolipoprotein AV; regeneration-associated protein 3; apolipoprotein A5
UniGene Number: N.A.
Accession Number: NP_443200
Source organism: human ( Homo sapiens )
Full length size (aa): To be posted
NCBI information page: click here

Full length sequence:
maavltwalallsafsatqarkgfwdyfsqtsgdkgrveqihqqkmarep
atlkdsleqdlnnmnkfleklrplsgseaprlpqdpvgmrrqlqeeleev
karlqpymaeahelvgwnleglrqqlkpytmdlmeqvalrvqelqeqlrv
vgedtkaqllggvdeawallqglqsrvvhhtgrfkelfhpyaeslvsgig
rhvqelhrsvaphapasparlsrcvqvlsrkltlkakalhariqqnldql
reelsrafagtgteegagpdpqmlseevrqrlqafrqdtylqiaaftrai
dqeteevqqqlappppghsafapefqqtdsgkvlsklqarlddlwedith
slhdqghshlgdp