human ( Homo sapiens )
CDT1

Western result: +

GST GST-CDT1 (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: CDT1
CBI Antibody Core Code: A1245
"Lot" Number: 1 Position: 100 (7-106)
% Identical Homology of Target to Mouse Protein: 41% (NP_080290.3| retroviral integration site 2; retroviral integration site 1)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
TDFFARRRPGPPRIAPPKLACRTPSPARPALRAPASATSG
SRKRARPPAAPGRDQARPPARRRLRLSVDEVSSPSTPEAP
DIPACPSPGQKIKKSTPAAG

Sequence Name: CDT1
Full name: CDT1: DNA replication factor
UniGene Number: Hs.122908
Accession Number: AAH09410
Source organism: human ( Homo sapiens )
Full length size (aa): 546
NCBI information page: click here

Full length sequence:
meqrrvtdffarrrpgppriappklacrtpsparpalrapasatsgsrkr
arppaapgrdqarpparrrlrlsvdevsspstpeapdipacpspgqkikk
stpaagqpphltsaqdqdtiselasclqrarelgarvralkasaqdages
ctpeaegrpeepcgekapayqrfhalaqpglpglvlpykyqvlaemfrsm
dtivgmlhnrsetptfakvqrgvqdmmrrrfeernvgqiktvypasyrfr
qersvptfkdgtrrsdyqltieplleqeadgaapqltasrllqrrqifsq
klvehvkehhkaflaslspamvvpedqltrwhprfnvdevpdiepaalpq
ppateklttaqevlararnlisprmekalsqlalrsaapsspgsprpalp
atppatppaaspsalkgvsqdllerirakeaqkqlaqmtrcpeqeqrlqr
lerlpelarvlrsvfvserkpalsmevacarmvgscctimspgemekhll
llsellpdwlslhrirtdtyvkldkaadlahitarlahqtraeegl