| Western result: ++ | | | | | | | | The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used. | | Antibody Name/Abbreviation: HPR CBI Antibody Core Code: 1Ab023 "Lot" Number: 1 Position: To be posted % Identical Homology of Target to Mouse Protein: N.A. Molecular Weight of GST-fusion: N.A. KDa Target sequence used to make this antibody: To be posted
| | Sequence Name: HPR Full name: UniGene Number: N.A. Accession Number: X89214 Source organism: human ( Homo sapiens ) Full length size (aa): 385 NCBI information page: click here Full length sequence: MHVCVCVCVCVYMPVCVDACMCCEAGRPAFRSFLFSLCSDLGAVISLLLW
GRQLFALYSGNDVTDISDDRFPKPPEIANGYVEHLFRYQCKNYYRLRTEG
DGVYTLNDKKQWINKAVGDKLPECEAVCGKPKNPANPVQRILGGHLDAKG
SFPWQAKMVSHHNLTTGATLINEQWLLTTAKNLFLNHSENATAKDIAPTL
TLYVGKKQLVEIEKVVLHPNYHQVDIGLIKLKQKVLVNERVMPICLPSKN
YAEVGRVGYVSGWGQSDNFKLTDHLKYVMLPVADQYDCITHYEGSTCPKW
KAPKSPVGVQPILNEHTFCVGMSKYQEDTCYGDAGSAFAVHDLEEDTWYA
AGILSFDKSCAVAEYGVYVKVTSIQHWVQKTIAEN
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