human ( Homo sapiens )
IgG3Ch

Antibody Name/Abbreviation: IgG3Ch-N
CBI Antibody Core Code: A0275
"Lot" Number: 2 Position: 100(1-100)
% Identical Homology of Target to Mouse Protein: 55% (XP_484178.1| similar to Ig gamma-2A chain C region secreted form (B allele))
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
ASTKGPSVFPLAPCSRSTSGGTAALGCLVKDYFPEPVTVS
WNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQT
YTCNVNHKPSNTKVDKRVEL

Other western:
Code: A0275
Lot: 1

Western result: ++

GST GST-IgG3Ch-N (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: IgG3Ch
Full name: IgG3 heavy chain constant region
UniGene Number: N.A.
Accession Number: X03604
Source organism: human ( Homo sapiens )
Full length size (aa): 377
NCBI information page: click here

Full length sequence:
ASTKGPSVFPLAPCSRSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGV
HTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYTCNVNHKPSNTKVDKRVEL
KTPLGDTTHTCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPRCPEPKSC
DTPPPCPRCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHED
PEVQFKWYVDGVEVHNAKTKPREEQYNSTFRVVSVLTVLHQDWLNGKEYK
CKVSNKALPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVSLTCLVK
GFYPSDIAVEWESSGQPENNYNTTPPMLDSDGSFFLYSKLTVDKSRWQQG
NIFSCSVMHEALHNRFTQKSLSLSPGK