human ( Homo sapiens )
KIAA0252

Western result: +

GST GST-KIAA0252 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: KIAA0252
CBI Antibody Core Code: A0605
"Lot" Number: 1 Position: 100(421-520)
% Identical Homology of Target to Mouse Protein: 91% (XP_283757.3| similar to KIAA0252 protein)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
AEALDRQRTKNISAISYINQRNREWNIVESEKALVAESHN
MKNQQMDPFTRRQCKPTIVSNSRDPAVQAAILAQLNAKYG
SGVLPDAPKEMSKGQGKDKD

Sequence Name: KIAA0252
Full name: KIAA0252 protein
UniGene Number: N.A.
Accession Number: BC015052
Source organism: human ( Homo sapiens )
Full length size (aa): 585
NCBI information page: click here

Full length sequence:
MKKQANKTASSGSSDKDSSAESSAPEEGEVSDSDSNSSSSSSDSDSSSED
EEFHDGYGEDLMGDEEDRARLEQMTEKEREQELFNRIEKREVLKRRFEIK
KKLKTAKKKEKKEKKKKQEEEQEKKKLTQIQESQVTSHNKERRSKRDEKL
DKKSQAMEELKAEREKRKNRTAELLAKKQPLKTSEVYSDDEEEEEDDKSS
EKSDRSSRTSSSDEEEEKEEIPPKSQPVSLPEELNRVRLSRHKLERWCHM
PFFAKTVTGCFVRIGIGNHNSKPVYRVAEITGVVETAKVYQLGGTRTNKG
LQLRHGNDQRVFRLEFVSNQEFTESEFMKWKEAMFSAGMQLPTLDEINKK
ELSIKEALNYKFNDQDIEEIVKEKERFRKAPPNYAMKKTQLLKEKAMAED
LGDQDKAKQIQDQLNELEERAEALDRQRTKNISAISYINQRNREWNIVES
EKALVAESHNMKNQQMDPFTRRQCKPTIVSNSRDPAVQAAILAQLNAKYG
SGVLPDAPKEMSKGQGKDKDLNSKSASDLSEDLFKVHDFDVKIDLQVPSS
ESKALAITSKAPPAKDGAPRRSLNLEDYKKRRGLI