human ( Homo sapiens )
KIAA0308

Western result: +

GST GST-KIAA0308 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: KIAA0308
CBI Antibody Core Code: A0704
"Lot" Number: 1 Position: 100(1699-1798)
% Identical Homology of Target to Mouse Protein: 94% (XP_284439.3| PREDICTED: RIKEN cDNA 1810014J18)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
SYTQPQGIPDTESPVPVINLKDGTRLAGDDAPKRKDLEKW
LKEHPGYVEDLGAFIPRMQLHEGRPKQKRHRCRNPNKLDV
NSLTGEERVQLINRRNARKV

Sequence Name: KIAA0308
Full name: KIAA0308
UniGene Number: N.A.
Accession Number: BAA20767
Source organism: human ( Homo sapiens )
Full length size (aa): 2119
NCBI information page: click here

Full length sequence:
EVSFCEDKDTGEPVIYYLVKWCSLPYEDSTWELKEDVDLAKIEEFEQLQA
SRPDTRRLDRPPSNIWKKIDQSRDYKNGNQLREYQLEGLNWLLFNWYNRR
NCILADEMGLGKTIQSITFLYEILLTGIRGPFLIIAPLSTIANWEREFRT
WTDINVVVYHGSLISRQMIQQYEMYFRDSQGRIIRGAYRFQAIITTFEMI
LGGCGELNAIEWRCVIIDEAHRLKNKNCKLLEGLKLMNLEHKVLLTGTPL
QNTVEELFSLLHFLEPLRFPSESTFMQEFGDLKTEEQVQKLQAILKPMML
RRLKEDVEKKLAPKEETIIEVELTNIQKKYYRAILEKNFSFLSKGAGQTN
VPNLVNTMMELRKCCNHPYLIKGAEEKILGEFRDTYNPAASDFHLQAMIQ
SAGKLVLIDKLLPKMKAGGHKVLIFSQMVRCLDILEDYLIHKRYLYERID
GRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSD
WNPQNDLQAQARCHRIGQNKAVKVYRLVTRNSYEREMFDRASLKLGLDKA
VLQSMSGRESNVGGIQQLSKKEIEDLLRRGAYGAIMEEEDEGSKFCEEDI
DQILLRRTKTITIESEGRGSTFAKASFVASGNRTDISLDDPNFWQKWAKK
AEIDIEAISGRNSLVIDTPRIRKQTRPFSATKDELAELSEAESEGDEKPK
LRRPCDRSNGYGRTECFRVEKNLLVYGWGRWREILSHGRFKRQLNEHDVE
IICRALLAYCLVHYRGDEKIKGFIWDLITPTEDGQTRELQNHLGLSAPVP
RGRKGKKVKTQTSSFDIQKAEWLRKYNPEQLLQDEGYKKHIKHHCNKVLL
RVRMLYYLKQEVIGNECQKVFDGVDASDIDVWVPEPDHSEVPAEWWDFDA
DKSLLIGVFKHGYEKYNTIRADPALCFLERVGKPDEKAVAAEQRANDYMD
GDVEDPEYKPAPAIFKDDIEDDVSSPGDLVIADGDGQLMEGDKVYWPTQS
ALTTRLRRLITAYQRTNKNRQIQQIQPTFSVPTSVMQPIYEEATLNPKMA
AKIERQQRWTRREEADFYRVVSTFGVVFDPDRGQFDWTKFRAMARLHKKT
DDSLEKYLYAFMSMCRRVCRLPSKEELVDPNIFIQPITEERASRTLYRIE
LLRKVREQALRHPQLFERLKLCHPNPDLPVWWECGPHDRDLLIGAAKHGV
SRTDYHILRDPELSFMAAQRNYSQSKMAHSRTSTPLLQQYQVALSASPLT
SLPRLLDAKGIILEEMKVKSENLKEEPQSSEEESMSSVETRTLIKSEPVS
PKNGVLPQATGDQKSGGKCETDRRMVAARTEPLTPNPASKKPRVHKRGSE
SSSDSDSDSERSSCSSRSSSSSSSSSCSHSRSGSSSSSSSSCSSASSSSS
SSTSSSSSSSSSSSEESDSDEEEAQKRAESTTHMKAYDEESVASLSTTQD
ETQDSFQMNNGTPESAYILQGGYMLAASYWPKDRVMINRLDSICQTVLKG
KWPSARRSYDANTVASFYTTKLLDSPGAATEYSEPSVPTPPGAGVKEEHD
QSTQMSKVKKHVREKEFTVKIKDEGGLKLTFQKQGLAQKRPFDGEDGALG
QQQYLTRLRELQSASETSLVNFPKSIPVSGTSIQPTLGANGVILDNQPIV
KKRRGRRKNVEGVDIFFFNRNKPPNHVSLGLTSSQISTGINPALSYTQPQ
GIPDTESPVPVINLKDGTRLAGDDAPKRKDLEKWLKEHPGYVEDLGAFIP
RMQLHEGRPKQKRHRCRNPNKLDVNSLTGEERVQLINRRNARKVGGAFAP
PLKDLCRFLKENSEYGVAPEWGDVVKQSGFLPESMYERILTGPVVREEVS
RRGRRPKSGIAKATAAAAAASATSVSGNPLLANGLLPGVDLTTLQALQQN
LQNLQSLQVTAGLMGMPTGLPSGGEAKNMAAMFPMLLSGMAGLPNLLGMG
GLLTKPTESGTEDKKGSDSKESEGKTERTESQSSENGGENSVSSSPSTSS
TAALNTAAAANPLALNPLLLSNILYPGMLLTPGLNLHIPTLSQSNTFDVQ
NKNSDLGSSKSVEVKEEDSRIKDQEDKGGTEPSPLNENSTDEGSEKADAS
SGSDSTSSSSEDSDSSNED