human ( Homo sapiens )
Leo1

Western result: +

GST GST-Leo1 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Leo1
CBI Antibody Core Code: A0603
"Lot" Number: 1 Position: 100(140-239)
% Identical Homology of Target to Mouse Protein: 85% (XP_134917.5| similar to senescence downregulated leo1-like)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
SDDEKWGREDKSDQSDDEKIQNSDDEERAQGSDEDKLQNS
DDDEKMQNTDDEERPQLSDDERQQLSEEEKANSDDERPVA
SDNDDEKQNSDDEEQPQLSD

Sequence Name: Leo1
Full name: Leo1 like protein
UniGene Number: N.A.
Accession Number: BC018147
Source organism: human ( Homo sapiens )
Full length size (aa): 666
NCBI information page: click here

Full length sequence:
MADMEDLFGSDADSEAERKDSDSGSDSDSDQENAASGSNASGSESDQDER
GDSGQPSNKELFGDDSEDEGASHHSGSDNHSERSDNRSEASERSDHEDND
PSDVDQHSGSEAPNDDEDEGHRSDGGSHHSEAEGSEKAHSDDEKWGREDK
SDQSDDEKIQNSDDEERAQGSDEDKLQNSDDDEKMQNTDDEERPQLSDDE
RQQLSEEEKANSDDERPVASDNDDEKQNSDDEEQPQLSDEEKMQNSDDER
PQASDEEHRHSDDEEEQDHKSESARGSDSEDEVLRMKRKNAIASDSEADS
DTEVPKDNSGTMDLFGGADDISSGSDGEDKPPTPGQPVDENGLPQDQQEE
EPIPETRIEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFED
EEMLDEEGRTRLKLKVENTIRWRIRRDEEGNEIKESNARIVKWSDGSMSL
HLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSAT
HRKMTLSLADRCSKTQKIRILPMAGRDPECQRTEMIKKEEERLRASIRRE
SQQRRMREKQHQRGLSASYLEPDRYDEEEEGEESISLAAIKNRYKGGIRE
ERARIYSSDSDEGSEEDKAQRLLKAKKLTSDEEGEPSGKRKAEDDDKANK
KHKKYVISDEEEEDDD