human ( Homo sapiens )
MNF

Western result: +

GST GST-MNF (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: MNF
CBI Antibody Core Code: A0775
"Lot" Number: 2 Position: 100(279-378)
% Identical Homology of Target to Mouse Protein: 94% (NP_951031.1| forkhead box K1 alpha isoform; myocyte nuclear factor)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
KRRQRGVSCFRTPFGPLSSRSAPASPTHPGLMSPRSGGLQ
TPECLSREGSPIPHDPEFGSKLASVPEYRYSQSAPGSPVS
AQPVIMAVPPRPSSLVAKPV

Sequence Name: MNF
Full name: MNF-myocyte nuclear Factor; similar to myocyte nuclear factor
UniGene Number: N.A.
Accession Number: XP_166528
Source organism: human ( Homo sapiens )
Full length size (aa): 616
NCBI information page: click here

Full length sequence:
MRQPSVTIGRNSSQGSVDLSMGLSSFISRRHLQLSFQEPHFYLRCLGKNG
VFVDGAFQRRGAPALQLPKQCTFRFPSTAIKIQFTSLYHKEEAPASPLRP
LYPQISPLKIHIPEPDLRSMVSPVPSPTGTISVPNSCPASPRGAGSSSYR
FVQNVTSDLQLAAEFAAKAASEQQADTSGGDSPKDESKPPFSYAQLIVQA
ISSAQDRQLTLSGIYAHITKHYPYYRTADKGWQNSIRHNLSLNRYFIKVP
RSQEEPGKGSFWRIDPASEAKLVEQAFRKRRQRGVSCFRTPFGPLSSRSA
PASPTHPGLMSPRSGGLQTPECLSREGSPIPHDPEFGSKLASVPEYRYSQ
SAPGSPVSAQPVIMAVPPRPSSLVAKPVAYMPASIVTSQQPAGHAIHVVQ
QAPTVTMVRVVTTSANSANGYILTSQGAAGGSHDAAGAAVLDLGSEARGL
EEKPTIAFATIPAAGGVIQTVASQMAPGVPGHTVTILQPATPVTLGQHHL
PVRAVTQNGKHAVPTNSLAGNAYALTSPLQLLATQASSSAPVVVTRVCEV
GPKEPAAAVAATATTTPATATTASASASSTGEPEVKRSRVEEPSGAVTTP
AGVIAAAGPQGPGTGE