human ( Homo sapiens )
NIRF

Western result: +

GST GST-NIRF (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: NIRF
CBI Antibody Core Code: A0180
"Lot" Number: 2 Position: 100(140-239)
% Identical Homology of Target to Mouse Protein: 91% (NP_659122.1| Np95-like ring finger protein; nuclear zinc finger protein Np97)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
LGAWFEAHIHSVTRASDGQSRGKTPLKNGSSCKRTNGNIK
HKSKENTNKLDSVPSTSNSDCVAADEDVIYHIQYDEYPES
GTLEMNVKDLRPRARTILKW

Sequence Name: NIRF
Full name: Np95-like ring finger protein
UniGene Number: N.A.
Accession Number: AB071698
Source organism: human ( Homo sapiens )
Full length size (aa): 802
NCBI information page: click here

Sequence Notes: ring finger (sometimes incorrectly labelled NIFR)

Full length sequence:
MWIQVRTIDGSKTCTIEDVSRKATIEELRERVWALFDVRPECQRLFYRGK
QLENGYTLFDYDVGLNDIIQLLVRPDPDHLPGTSTQIEAKPCSNSPPKVK
KAPRVGPSNQPSTSARARLIDPGFGIYKVNELVDARDVGLGAWFEAHIHS
VTRASDGQSRGKTPLKNGSSCKRTNGNIKHKSKENTNKLDSVPSTSNSDC
VAADEDVIYHIQYDEYPESGTLEMNVKDLRPRARTILKWNELNVGDVVMV
NYNVESPGQRGFWFDAEITTLKTISRTKKELRVKIFLGGSEGTLNDCKII
SVDEIFKIERPGAHPLSFADGKFLRRNDPECDLCGGDPEKKCHSCSCRVC
GGKHEPNMQLLCDECNVAYHIYCLNPPLDKVPEEEYWYCPSCKTDSSEVV
KAGERLKMSKKKAKMPSASTESRRDWGRGMACVGRTRECTIVPSNHYGPI
PGIPVGSTWRFRVQVSEAGVHRPHVGGIHGRSNDGAYSLVLAGGFADEVD
RGDEFTYTGSGGKNLAGNKRIGAPSADQTLTNMNRALALNCDAPLDDKIG
AESRNWRAGKPVRVIRSFKGRKISKYAPEEGNRYDGIYKVVKYWPEISSS
HGFLVWRYLLRRDDVEPAPWTSEGIERSRRLCLRLQYPAGYPSDKEGKKP
KGQSKKQPSGTTKRPISDDDCPSASKVYKASDSAEAIEAFQLTPQQQHLI
REDCQNQKLWDEVLSHLVEGPNFLKKLEQSFMCVCCQELVYQPVTTECFH
NVCKDCLQRSFKAQVFSCPACRHDLGQNYIMIPNEILQTLLDLFFPGYSK
GR