human ( Homo sapiens )
PARG

Western result: +

GST GST-PARG (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: PARG
CBI Antibody Core Code: A0618
"Lot" Number: 1 Position: 100(267-366)
% Identical Homology of Target to Mouse Protein: 68% (NP_036090.1| poly (ADP-ribose) glycohydrolase)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
GSEDVGTGPKNDNKLTRQESCLGNSPPFEKESEPESPMDV
DNSKNSCQDSEADEETSPGFDEQEDGSSSQTANKPSRFQA
RDADIEFRKRYSTKGGEVRL

Sequence Name: PARG
Full name: poly (ADP-ribose) glycohydrolase
UniGene Number: N.A.
Accession Number: BC052966
Source organism: human ( Homo sapiens )
Full length size (aa): 976
NCBI information page: click here

Full length sequence:
MNAGPGCEPCTKRPRWGAATTSPAASDARSFPSRQRRVLDPKDAHVQFRV
PPSSPACVPGRAGQHRGSATSLVFKQKTITSWMDTKGIKTAESESLDSKE
NNNTRIESMMSSVQKDNFYQHNVEKLENVSQLSLDKSPTEKSTQYLNQHQ
TAAMCKWQNEGKHTEQLLESEPQTVTLVPEQFSNANIDRSPQNDDHSDTD
SEENRDNQQFLTTVKLANAKQTTEDEQAREAKSHQKCSKSCDPGEDCASC
QQDEIDVVPESPLSDVGSEDVGTGPKNDNKLTRQESCLGNSPPFEKESEP
ESPMDVDNSKNSCQDSEADEETSPGFDEQEDGSSSQTANKPSRFQARDAD
IEFRKRYSTKGGEVRLHFQFEGGESRTGMNDLNAKLPGNISSLNVECRNS
KQHGKKDSKITDHFMRLPKAEDRRKEQWETKHQRTERKIPKYVPPHLSPD
KKWLGTPIEEMRRMPRCGIRLPLLRPSANHTVTIRVDLLRAGEVPKPFPT
HYKDLWDNKHVKMPCSEQNLYPVEDENGERTAGSRWELIQTALLNKFTRP
QNLKDAILKYNVAYSKKWDFTALIDFWDKVLEEAEAQHLYQSILPDMVKI
ALCLPNICTQPIPLLKQKMNHSITMSQEQIASLLANAFFCTFPRRNAKMK
SEYSSYPDINFNRLFEGRSSRKPEKLKTLFCYFRRVTEKKPTGLVTFTRQ
SLEDFPEWERCEKPLTRLHVTYEGTIEENGQGMLQVDFANRFVGGGVTSA
GLVQEEIRFLINPELIISRLFTEVLDHNECLIITGTEQYSEYTGYAETYR
WSRSHEDGSERDDWQRHCTEIVAIDALHFRRYLDQFVPEKMRRELNKAYC
GFLRPGVSSENLSAVATGNWGCGAFGGDARLKALIQILAAAAAERDVVYF
TFGDSELMRDIYSMHIFLTERKLTVGDVYKLLLRYYNEECRNCSTPGPDI
KLYPFIYHAVESCAETADHSGQRTGT