human ( Homo sapiens )
PC-1 (ENPP1)

Western result: +

GST GST-PC-1 (ENPP1) (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: PC-1 (ENPP1)
CBI Antibody Core Code: A0344
"Lot" Number: 2 Position: 100(774-873
% Identical Homology of Target to Mouse Protein: 75% (NP_032839.2| ectonucleotide pyrophosphatase/phosphodiesterase 1; phosphodiesterase I/nucleotide pyrophosphatase 1; 1 ectonucleotide pyrophosphatase/phosphodiesterase 1; tiptoe walking)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
LIPTHFFIVLTSCKDTSQTPLHCENLDTLAFILPHRTDNS
ESCVHGKHDSSWVEELLMLHRARITDVEHITGLSFYQQRK
EPVSDILKLKTHLPTFSQED

Sequence Name: PC-1 (ENPP1)
Full name: Ectonucleotide pyrophosphatase/phosphodiesterase 1
UniGene Number: N.A.
Accession Number: NM_006208
Source organism: human ( Homo sapiens )
Full length size (aa): 873
NCBI information page: click here

Full length sequence:
MDVGEEPLEKAARARTAKDPNTYKVLSLVLSVCVLTTILGCIFGLKPSCA
KEVKSCKGRCFERTFGNCRCDAACVELGNCCLDYQETCIEPEHIWTCNKF
RCGEKRLTRSLCACSDDCKDKGDCCINYSSVCQGEKSWVEEPCESINEPQ
CPAGFETPPTLLFSLDGFRAEYLHTWGGLLPVISKLKKCGTYTKNMRPVY
PTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPEWYK
GEPIWVTAKYQGLKSGTFFWPGSDVEINGIFPDIYKMYNGSVPFEERILA
VLQWLQLPKDERPHFYTLYLEEPDSSGHSYGPVSSEVIKALQRVDGMVGM
LMDGLKELNLHRCLNLILISDHGMEQGSCKKYIYLNKYLGDVKNIKVIYG
PAARLRPSDVPDKYYSFNYEGIARNLSCREPNQHFKPYLKHFLPKRLHFA
KSDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNVFSNMQALFVGYG
PGFKHGIEADTFENIEVYNLMCDLLNLTPAPNNGTHGSLNHLLKNPVYTP
KHPKEVHPLVQCPFTRNPRDNLGCSCNPSILPIEDFQTQFNLTVAEEKII
KHETLPYGRPRVLQKENTICLLSQHQFMSGYSQDILMPLWTSYTVDRNDS
FSTEDFSNCLYQDFRIPLSPVHKCSFYKNNTKVSYGFLSPPQLNKNSSGI
YSEALLTTNIVPMYQSFQVIWRYFHDTLLRKYAEERNGVNVVSGPVFDFD
YDGRCDSLENLRQKRRVIRNQEILIPTHFFIVLTSCKDTSQTPLHCENLD
TLAFILPHRTDNSESCVHGKHDSSWVEELLMLHRARITDVEHITGLSFYQ
QRKEPVSDILKLKTHLPTFSQED