human ( Homo sapiens )
PDIR (ERP70)

Antibody Name/Abbreviation: PDIR (ERP70)
CBI Antibody Core Code: A0293
"Lot" Number: 2 Position: 100(520-619)
% Identical Homology of Target to Mouse Protein: 64% (NP_031978.1| glucose regulated protein; endoplasmic reticulum protein; glucose regulated protein, 58 kDa; phospholipase C, alpha)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
PKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCK
QLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEG
FPTIYFAPSGDKKNPVKFEG

Other western:
Code: A0293
Lot: 1

Western result: ++

GST GST-PDIR (ERP70) (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: PDIR (ERP70)
Full name: Protein disulfide isomerase related protein (calcium binding)
UniGene Number: N.A.
Accession Number: NP_004902.1
Source organism: human ( Homo sapiens )
Full length size (aa): 645
NCBI information page: click here

Full length sequence:
MRPRKAFLLLLLLGLVQLLAVAGAEGPDEDSSNRENAIEDEEEEEEEDDD
EEEDDLEVKEENGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPE
YEKIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKKGQAVD
YEGSRTQEEIVAKVREVSQPDWTPPPEVTLVLTKENFDEVVNDADIILVE
FYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAETDLAKRFDVS
GYPTLKIFRKGRPYDYNGPREKYGIVDYMIEQSGPPSKEILTLKQVQEFL
KDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKV
SQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHR
KVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTF
AIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLRE
FVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEF
YAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGF
PTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKEEL