human ( Homo sapiens )
PNUTS

Western result: +

GST GST-PNUTS (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: PNUTS
CBI Antibody Core Code: A0643
"Lot" Number: 1 Position: 100(620-719)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
LGPGPIANGFPPGGPGGPKGMQHFPPGPGGPMPGPHGGPG
GPVGPRLLGPPPPPRGGDPFWDGPGDPMRGGPMRGGPGPG
PGPYHRGRGGRGGNEPPPPP

Sequence Name: PNUTS
Full name: Homo sapiens protein phosphatase 1, regulatory subunit 10
UniGene Number: N.A.
Accession Number: NM_002714
Source organism: human ( Homo sapiens )
Full length size (aa): 940
NCBI information page: click here

Full length sequence:
MGSGPIDPKELLKGLDSFLNRDGEVKSVDGISKIFSLMKEARKMVSRCTY
LNILLQTRSPEILVKFIDVGGYKLLNNWLTYSKTTNNIPLLQQILLTLQH
LPLTVDHLKQNNTAKLVKQLSKSSEDEELRKLASVLVSDWMAVIRSQSST
QPAEKDKKKRKDEGKSRTTLPERPLTEVKAETRAEEAPEKKREKPKSLRT
TAPSHAKFRSTGLELETPSLVPVKKNASTVVVSDKYNLKPIPLKRQSNVA
APGDATPPAEKKYKPLNTTPNATKEIKVKIIPPQPMEGLGFLDALNSAPV
PGIKIKKKKKVLSPTAAKPSPFEGKTSTEPSTAKPSSPEPAPPSEAMDAD
RPGTPVPPVEVPELMDTASLEPGALDAKPVESPGDPNQLTRKGRKRKSVT
WPEEGKLREYFYFELDETERVNVNKIKDFGEAAKREILSDRHAFETARRL
SHDNMEEKVPWVCPRPLVLPSPLVTPGSNSQERYIQAEREKGILQELFLN
KESPHEPDPEPYEPIPPKLIPLDEECSMDETPYVETLEPGGSGGSPDGAG
GSKLPPVLANLMGSMGAGKGPQGPGGGGINVQEILTSIMGSPNSHPSEEL
LKQPDYSDKIKQMLVPHGLLGPGPIANGFPPGGPGGPKGMQHFPPGPGGP
MPGPHGGPGGPVGPRLLGPPPPPRGGDPFWDGPGDPMRGGPMRGGPGPGP
GPYHRGRGGRGGNEPPPPPPPFRGARGGRSGGGPPNGRGGPGGGMVGGGG
HRPHEGPGGGMGNSSGHRPHEGPGGGMGSGHRPHEGPGGSMGGGGGHRPH
EGPGGGISGGSGHRPHEGPGGGMGAGGGHRPHEGPGGSMGGSGGHRPHEG
PGHGGPHGHRPHDVPGHRGHDHRGPPPHEHRGHDGPGHGGGGHRGHDGGH
SHGGDMSNRPVCRHFMMKGNCRYENNCAFYHPGVNGPPLP