human ( Homo sapiens )
Rad54

Western result: +

GST GST-Rad54 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Rad54
CBI Antibody Core Code: A0708
"Lot" Number: 1 Position: 100(11-110)
% Identical Homology of Target to Mouse Protein: 90% (NP_033041.2| RAD54 like)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
AKRKPEGRSCDDEDWQPGLVTPRKRKSSSETQIQECFLSP
FRKPLSQLTNQPPCLDSSQHEAFIRSILSKPFKVPIPNYQ
GPLGSRALGLKRAGVRRALH

Sequence Name: Rad54
Full name:
UniGene Number: N.A.
Accession Number: CAA66379
Source organism: human ( Homo sapiens )
Full length size (aa): 747
NCBI information page: click here

Full length sequence:
MRRSLAPSQLAKRKPEGRSCDDEDWQPGLVTPRKRKSSSETQIQECFLSP
FRKPLSQLTNQPPCLDSSQHEAFIRSILSKPFKVPIPNYQGPLGSRALGL
KRAGVRRALHDPLEKDALVLYEPPPLSAHDQLKLDKEKLPVHVVVDPILS
KVLRPHQREGVKFLWECVTSRRIPGSHGCIMADEMGLGKTLQCITLMWTL
LRQSPECKPEIDKAVVVSPSSLVKNWYNEVGKWLGGRIQPLAIDGGSKDE
IDQKLEGFMNQRGARVSSPILIISYETFRLHVGVLQKGSVGLVICDEGHR
LKNSENQTYQALDSLNTSRRVLISGTPIQNDLLEYFSLVHFVNSGILGTA
HEFKKHFELPILKGRDAAASEADRQLGEERLRELTSIVNRCLIRRTSDIL
SKYLPVKIEQVVCCRLTPLQTELYKRFLRQAKPAEELLEGKMSVSSLSSI
TSLKKLCNHPALIYDKCVEEEDGFVGALDLFPPGYSSKALEPQLSGKMLV
LDYILAVTRSRSSDKVVLVSNYTQTLDLFEKLCRARRYLYVRLDGTMSIK
KRAKVVERFNSPSSPDFVFMLSSKAGGCGLNLIGANRLVMFDPDWNPAND
EQAMARVWRDGQKKTCYIYRLLSAGTIEEKIFQRQSHKKALSSCVVDEEQ
DVERHFSLGELKELFILDEASLSDTHDRLHCRRCVNSRQIRPPPDGSDCT
SDLAGWNHCTDKWGLRDEVLQAAWDAASTAITFVFHQHSHEEQRGLR