human ( Homo sapiens )
S1

Western result: +/-

GST GST-S1 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: S1
CBI Antibody Core Code: A0438
"Lot" Number: 1 Position: 100(854-953)
% Identical Homology of Target to Mouse Protein: 29% (NP_031660.2| cyclin F)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
MEVDEAEKKEEKEKKKEPEPNFQLLDNPARVMPAQLKVLT
MPETCRYQPFKPLSIGGIIILKDTSEDIEELVEPVAAHGP
KIEEEEQEPEPPEPFEYIDD

Sequence Name: S1
Full name: proteasome 26S non-ATPase subunit 1
UniGene Number: N.A.
Accession Number: NP_002798
Source organism: human ( Homo sapiens )
Full length size (aa): 953
NCBI information page: click here

Full length sequence:
MITSAAGIISLLDEDEPQLKEFALHKLNAVVNDFWAEISESVDKIEVLYE
DEGFRSRQFAALVASKVFYHLGAFEESLNYALGAGDLFNVNDNSEYVETI
IAKCIDHYTKQCVENADLPEGEKKPIDQRLEGIVNKMFQRCLDDHKYKQA
IGIALETRRLDVFEKTILESNDVPGMLAYSLKLCMSLMQNKQFRNKVLRV
LVKIYMNLEKPDFINVCQCLIFLDDPQAVSDILEKLVKEDNLLMAYQICF
DLYESASQQFLSSVIQNLRTVGTPIASVPGSTNTGTVPGSEKDSDSMETE
EKTSSAFVGKTPEASPEPKDQTLKMIKILSGEMAIELHLQFLIRNNNTDL
MILKNTKDAVRNSVCHTATVIANSFMHCGTTSDQFLRDNLEWLARATNWA
KFTATASLGVIHKGHEKEALQLMATYLPKDTSPGSAYQEGGGLYALGLIH
ANHGGDIIDYLLNQLKNASNDIVRHGGSLGLGLAAMGTARQDVYDLLKTN
LYQDDAVTGEAAGLALGLVMLGSKNAQAIEDMVGYAQETQHEKILRGLAV
GIALVMYGRMEEADALIESLCRDKDPILRRSGMYTVAMAYCGSGNNKAIR
RLLHVAVSDVNDDVRRAAVESLGFILFRTPEQCPSVVSLLSESYNPHVRY
GAAMALGICCAGTGNKEAINLLEPMTNDPVNYVRQGALIASALIMIQQTE
ITCPKVNQFRQLYSKVINDKHDDVMAKFGAILAQGILDAGGHNVTISLQS
RTGHTHMPSVVGVLVFTQFWFWFPLSHFLSLAYTPTCVIGLNKDLKMPKV
QYKSNCKPSTFAYPAPLEVPKEKEKEKVSTAVLSITAKAKKKEKEKEKKE
EEKMEVDEAEKKEEKEKKKEPEPNFQLLDNPARVMPAQLKVLTMPETCRY
QPFKPLSIGGIIILKDTSEDIEELVEPVAAHGPKIEEEEQEPEPPEPFEY
IDD