human ( Homo sapiens )
S2

Western result: +

control S2 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: S2
CBI Antibody Core Code: A0439
"Lot" Number: 1 Position: 20(618-637)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
EHFDSKEKEEDKDKKEKKDK

Sequence Name: S2
Full name: proteasome 26S non-ATPase subunit 2
UniGene Number: N.A.
Accession Number: NP_002799
Source organism: human ( Homo sapiens )
Full length size (aa): 908
NCBI information page: click here

Full length sequence:
MEEGGRDKAPVQPQQSPAAAPGGTDEKPSGKERRDAGDKDKEQELSEEDK
QLQDELEMLVERLGEKDTSLYRPALEELRRQIRSSTTSMTSVPKPLKFLR
PHYGKLKEIYENMAPGENKRFAADIISVLAMTMSGERECLKYRLVGSQEE
LASWGHEYVRHLAGEVAKEWQELDDAEKVQREPLLTLVKEIVPYNMAHNA
EHEACDLLMEIEQVDMLEKDIDENAYAKVCLYLTSCVNYVPEPENSALLR
CALGVFRKFSRFPEALRLALMLNDMELVEDIFTSCKDVVVQKQMAFMLGR
HGVFLELSEDVEEYEDLTEIMSNVQLNSNFLALARELDIMEPKVPDDIYK
THLENNRFGGSGSQVDSARMNLASSFVNGFVNAAFGQDKLLTDDGNKWLY
KNKDHGMLSAAASLGMILLWDVDGGLTQIDKYLYSSEDYIKSGALLACGI
VNSGVRNECDPALALLSDYVLHNSNTMRLGSIFGLGLAYAGSNREDVLTL
LLPVMGDSKSSMEVAGVTALACGMIAVGSCNGDVTSTILQTIMEKSETEL
KDTYARWLPLGLGLNHLGKGEAIEAILAALEVVSEPFRSFANTLVDVCAY
AGSGNVLKVQQLLHICSEHFDSKEKEEDKDKKEKKDKDKKEAPADMGAHQ
GVAVLGIALIAMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVS
NPRLNILDTLSKFSHDADPEVSYNSIFAMGMVGSGTNNARLAAMLRQLAQ
YHAKDPNNLFMVRLAQGLTHLGKGTLTLCPYHSDRQLMSQVAVAGLLTVL
VSFLDVRNIILGKSHYVLYGLVAAMQPRMLVTFDEELRPLPVSVRVGQAV
DVVGQAGKPKTITGFQTHTTPVLLAHGERAELATEEFLPVTPILEGFVIL
RKNPNYDL