| Western result: + | | | | | | | | The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST. | | Antibody Name/Abbreviation: sds22 CBI Antibody Core Code: A0644 "Lot" Number: 1 Position: 100(2-101) % Identical Homology of Target to Mouse Protein: 66% (XP_489811.1| similar to protein phosphatase-1 regulatory subunit 7) Molecular Weight of fusion protein: 38 KDa Target sequence used to make this antibody: AAERGAGQQQSQEMMEVDRRVESEESGDEEGKKHSSGIVA
DLSEQSLKDGEERGEEDPEEEHELPVDMETINLDRDAEDV
DLNHYRIGKIEGFEVLKKVK
| | Sequence Name: sds22 Full name: Homo sapiens protein phosphatase 1, regulatory subunit 7 (PPP1R7) UniGene Number: N.A. Accession Number: NM_002712 Source organism: human ( Homo sapiens ) Full length size (aa): 360 NCBI information page: click here Full length sequence: MAAERGAGQQQSQEMMEVDRRVESEESGDEEGKKHSSGIVADLSEQSLKD
GEERGEEDPEEEHELPVDMETINLDRDAEDVDLNHYRIGKIEGFEVLKKV
KTLCLRQNLIKCIENLEELQSLRELDLYDNQIKKIENLEALTELEILDIS
FNLLRNIEGVDKLTRLKKLFLVNNKISKIENLSNLHQLQMLELGSNRIRA
IENIDTLTNLESLFLGKNKITKLQNLDALTNLTVLSMQSNRLTKIEGLQN
LVNLRELYLSHNGIEVIEGLENNNKLTMLDIASNRIKKIENISHLTELQE
FWMNDNLLESWSDLDELKGARSLETVYLERNPLQKDPQYRRKVMLALPSV
RQIDATFVRF
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