human ( Homo sapiens )
SerPIN

Antibody Name/Abbreviation: SerPIN
CBI Antibody Core Code: A0285
"Lot" Number: 2 Position: 100(106-205)
% Identical Homology of Target to Mouse Protein: 83% (NP_766640.1| serine (or cysteine) proteinase inhibitor, clade B, member 1b; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1b)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
VSTQKTYGADLASVDFQHASEDARKTINQWVKGQTEGKIP
ELLASGMVDNMTKLVLVNAIYFKGNWKDKFMKEATTNAPF
RLNKKDRKTVKMMYQKKKFA

Other western:
Code: A0285
Lot: 1

Western result: +

GST GST-SerPIN (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: SerPIN
Full name: Neutrophil protease inhibitor
UniGene Number: N.A.
Accession Number: NP_109591.1
Source organism: human ( Homo sapiens )
Full length size (aa): 379
NCBI information page: click here

Full length sequence:
MEQLSSANTRFALDLFLALSENNPAGNIFISPFSISSAMAMVFLGTRGNT
AAQLSKTFHFNTVEEVHSRFQSLNADINKRGASYILKLANRLYGEKTYNF
LPEFLVSTQKTYGADLASVDFQHASEDARKTINQWVKGQTEGKIPELLAS
GMVDNMTKLVLVNAIYFKGNWKDKFMKEATTNAPFRLNKKDRKTVKMMYQ
KKKFAYGYIEDLKCRVLELPYQGEELSMVILLPDDIEDESTGLKKIEEQL
TLEKLHEWTKPENLDFIEVNVSLPRFKLEESYTLNSDLARLGVQDLFNSS
KADLSGMSGARDIFISKIVHKSFVEVNEEGTEAAAATAGIATFCMLMPEE
NFTADHPFLFFIRHNSSGSILFLGRFSSP