human ( Homo sapiens )
THBD

Western result: +-

GST GST-THBD (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: THBD
CBI Antibody Core Code: A0541
"Lot" Number: 1 Position: 100(16-115)
% Identical Homology of Target to Mouse Protein: 71% (NP_033404.1| thrombomodulin)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
GFPAPAEPQPGGSQCVEHDCFALYPGPATFLNASQICDGL
RGHLMTVRSSVAADVISLLLNGDGGVGRRRLWIGLQLPPG
CGDPKRLGPLRGFQWVTGDN

Sequence Name: THBD
Full name: Thrombomodulin precursor (Fetomodulin) (TM) (CD141 antigen).
UniGene Number: Hs.2030
Accession Number: P07204
Source organism: human ( Homo sapiens )
Full length size (aa): 575
NCBI information page: click here

Full length sequence:
MLGVLVLGALALAGLGFPAPAEPQPGGSQCVEHDCFALYPGPATFLNASQ
ICDGLRGHLMTVRSSVAADVISLLLNGDGGVGRRRLWIGLQLPPGCGDPK
RLGPLRGFQWVTGDNNTSYSRWARLDLNGAPLCGPLCVAVSAAEATVPSE
PIWEEQQCEVKADGFLCEFHFPATCRPLAVEPGAAAAAVSITYGTPFAAR
GADFQALPVGSSAAVAPLGLQLMCTAPPGAVQGHWAREAPGAWDCSVENG
GCEHACNAIPGAPRCQCPAGAALQADGRSCTASATQSCNDLCEHFCVPNP
DQPGSYSCMCETGYRLAADQHRCEDVDDCILEPSPCPQRCVNTQGGFECH
CYPNYDLVDGECVEPVDPCFRANCEYQCQPLNQTSYLCVCAEGFAPIPHE
PHRCQMFCNQTACPADCDPNTQASCECPEGYILDDGFICTDIDECENGGF
CSGVCHNLPGTFECICGPDSALARHIGTDCDSGKVDGGDSGSGEPPPSPT
PGSTLTPPAVGLVHSGLLIGISIASLCLVVALLALLCHLRKKQGAARAKM
EYKCAAPSKEVVLQHVRTERTPQRL