human ( Homo sapiens )
UCH37

Western result: +

GST GST-UCH37 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: UCH37
CBI Antibody Core Code: A0787
"Lot" Number: 1 Position: 100(196-295)
% Identical Homology of Target to Mouse Protein: 95% (NP_062508.1| ubiquitin C-terminal hydrolase 37; biquitin carboxyl-terminal esterase L5)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
WISAVRPVIEKRIQKYSEGEIRFNLMAIVSDRKMIYEQKI
AELQRQLAEEEPMDTDQGNSMLSAIQSEVAKNQMLIEEEV
QKLKRYKIENIRRKHNYLPF

Sequence Name: UCH37
Full name: ubiquitin carboxyl-terminal hydrolase L5
UniGene Number: Hs.187357
Accession Number: NM_015984
Source organism: human ( Homo sapiens )
Full length size (aa): 329
NCBI information page: click here

Full length sequence:
MTGNAGEWCLMESDPGVFTELIKGFGCRGAQVEEIWSLEPENFEKLKPVH
GLIFLFKWQPGEEPAGSVVQDSRLDTIFFAKQVINNACATQAIVSVLLNC
THQDVHLGETLSEFKEFSQSFDAAMKGLALSNSDVIRQVHNSFARQQMFE
FDTKTSAKEEDAFHFVSYVPVNGRLYELDGLREGPIDLGACNQDDWISAV
RPVIEKRIQKYSEGEIRFNLMAIVSDRKMIYEQKIAELQRQLAEEEPMDT
DQGNSMLSAIQSEVAKNQMLIEEEVQKLKRYKIENIRRKHNYLPFIMELL
KTLAEHQQLIPLVEKAKEKQNAKKAQETK