human ( Homo sapiens )
UCRP

Western result: +-

GST GST-UCRP (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: UCRP
CBI Antibody Core Code: A0025
"Lot" Number: 1 Position: To be posted
% Identical Homology of Target to Mouse Protein: 60% (NP_056598.1| interferon, alpha-inducible protein; Interferon-induced 15-KDa protein; interferon-stimulated protein (15 kDa); interferon-stimulated protein)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
MGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHA
FQQRLAVHPSGVALQDRVPLASQGLGPGSTVLLVVDKCDE
PLSILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQDD
LFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGGGTE
PGGRS

Sequence Name: UCRP
Full name: hCRP?
UniGene Number: N.A.
Accession Number: M13755
Source organism: human ( Homo sapiens )
Full length size (aa): To be posted
NCBI information page: click here

Full length sequence:
MGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRLAVHPS
GVALQDRVPLASQGLGPGSTVLLVVDKCDEPLSILVRNNKGRSSTYEVRL
TQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPLSTVFM
NLRLRGGGTEPGGRS