human ( Homo sapiens )
Unknown

Western result: +

GST GST-Unknown (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Unknown
CBI Antibody Core Code: A0694
"Lot" Number: 1 Position: 100(228-327)
% Identical Homology of Target to Mouse Protein: 58% (NP_031981.2| protein tyrosine phosphatase, receptor type, V; embryonic stem cell phosphatase)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
VGRAERTRTGSWGTLSAFSLSNPPTRSWPISVTDLPQACA
KRAASANAGFLKEYEAIKDEAGFSAPPPGYEQDSPVSYDR
SQGQFSPVEESPPDDMPEAW

Sequence Name: Unknown
Full name: similar to Osteotesticular phosphatase; protein tyrosine phosphatase, receptor type, W (LOC347319)
UniGene Number: N.A.
Accession Number: XM_294600
Source organism: human ( Homo sapiens )
Full length size (aa): 538
NCBI information page: click here

Full length sequence:
MGAGGFMCPTLNAQLSLSADDHSRVRLTQLEGEPHSDYINANLVPGYTRP
QEFIASQGPLKKTLENFWRLVREQQVRIIIMPTISMENGRVLCEHYWLTD
STPDTHGHITIHLLAEEPEDEWTKREFQLQHAPSSLLAFMELLLRGCPCG
VGMGRTGTFVALSRLLQQLEEEQMVDVFHAVYALRMHQPLMIQTLGLKNL
PAGPPRSSLSSLMPFPLAEPAGSLLLFVGRAERTRTGSWGTLSAFSLSNP
PTRSWPISVTDLPQACAKRAASANAGFLKEYEAIKDEAGFSAPPPGYEQD
SPVSYDRSQGQFSPVEESPPDDMPEAWLFPGGPSGRDHTVLTGPAGPKEL
WELVWQHRAHVLVSLCPPNVMEKEFWPTEMQPVVTDMVTVHWVAESSTAG
WFCTLLRVTHGESRKEREVQRLQFPYLEPGHELPATTLLPFLAAVGQCCS
RGNNKKPGTLLSHSKCCWTMVSCSKGATQLGTFLAMEQLLQQAGSECTVD
IFNVALQQSQACGLMTPTLKQYVYLYNCLNSALADGLP