human ( Homo sapiens )
C-myb

Western result: +

GST GST-C-myb (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: C-myb
CBI Antibody Core Code: A0242
"Lot" Number: 2 Position: 100(1-100)
% Identical Homology of Target to Mouse Protein: 100% (NP_291075.1| myeloblastosis proto-oncogene product; myb protein)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
QRVIELVQKYGPKRWSVIAKHLKGRIGKQCRERWHNHLNP
EVKKTSWTEEEDRIIYQAHKRLGNRWAEIAKLLPGRTDNA
IKNHWNSTMRRKVEQEGYLQ

Other western:
Code: A0242
Lot: 1

Western result: +

GST GST-C-myb (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: C-myb
Full name:
UniGene Number: N.A.
Accession Number: M15024
Source organism: human ( Homo sapiens )
Full length size (aa): 640
NCBI information page: click here

Full length sequence:
MARRPRHSIYSSDEDDEDFEMCDHDYDGLLPKSGKRHLGKTRWTREEDEK
LKKLVEQNGTDDWKVIANYLPNRTDVQCQHRWQKVLNPELIKGPWTKEED
QRVIELVQKYGPKRWSVIAKHLKGRIGKQCRERWHNHLNPEVKKTSWTEE
EDRIIYQAHKRLGNRWAEIAKLLPGRTDNAIKNHWNSTMRRKVEQEGYLQ
ESSKASQPAVATSFQKNSHLMGFAQAPPTAQLPATGQPTVNNDYSYYHIS
EAQNVSSHVPYPVALHVNIVNVPQPAAAAIQRHYNDEDPEKEKRIKELEL
LLMSTENELKGQQVLPTQNHTCSYPGWHSTTIADHTRPHGDSAPVSCLGE
HHSTPSLPADPGSLPEESASPARCMIVHQGTILDNVKNLLEFAETLQFID
SFLNTSSNHENSDLEMPSLTSTPLIGHKLTVTTPFHRDQTVKTQKENTVF
RTPAIKRSILESSPRTPTPFKHALAAQEIKYGPLKMLPQTPSHLVEDLQD
VIKQESDESGFVAEFQENGPPLLKKIKQEVESPTDKSGNFFCSHHWEGDS
LNTQLFTQTSPVRDAPNILTSSVLMAPASEDEDNVLKAFTVPKNRSLASP
LQPCSSTWEPASCGKMEEQMTSSSQARKYVNAFSARTLVM