human ( Homo sapiens )
Caspase 1

Western result: ++

GST GST-Caspase 1 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Caspase 1
CBI Antibody Core Code: 1Ab011
"Lot" Number: 1 Position: To be posted
% Identical Homology of Target to Mouse Protein: 70% (NP_033937.1| caspase 1; IL-1B converting enzyme; interleukin 1 beta-converting enzyme)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
LEAFAHRPEHKTSDSTFLVFMSHGIREGICGKKHSEQVPD
ILQLNAIFNMLNTKNCPSLKDKPKVIIIQACRGDSPGVVW
FKDSVGVSGNLSLPTTEEFE

Sequence Name: Caspase 1
Full name:
UniGene Number: N.A.
Accession Number: NM_033292
Source organism: human ( Homo sapiens )
Full length size (aa): 404
NCBI information page: click here

Full length sequence:
MADKVLKEKRKLFIRSMGEGTINGLLDELLQTRVLNKEEMEKVKRENATV
MDKTRALIDSVIPKGAQACQICITYICEEDSYLAGTLGLSADQTSGNYLN
MQDSQGVLSSFPAPQAVQDNPAMPTSSGSEGNVKLCSLEEAQRIWKQKSA
EIYPIMDKSSRTRLALIICNEEFDSIPRRTGAEVDITGMTMLLQNLGYSV
DVKKNLTASDMTTELEAFAHRPEHKTSDSTFLVFMSHGIREGICGKKHSE
QVPDILQLNAIFNMLNTKNCPSLKDKPKVIIIQACRGDSPGVVWFKDSVG
VSGNLSLPTTEEFEDDAIKKAHIEKDFIAFCSSTPDNVSWRHPTMGSVFI
GRLIEHMQEYACSCDVEEIFRKVRFSFEQPDGRAQMPTTERVTLTRCFYL
FPGH