human ( Homo sapiens )
CRSP2BP

Western result: ++

GST GST-CRSP2BP (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: CRSP2BP
CBI Antibody Core Code: A0138
"Lot" Number: 1 Position: 100(13-112)
% Identical Homology of Target to Mouse Protein: 77% (NP_852082.1| cysteine and glycine-rich protein 2 binding protein; cysteine-rich protein 2 binding protein)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
RHDDEATRTSTSEGLEEGEVEGETLLIVESEDQASVDLSH
DQSGDSLNSDEGDVSWMEEQLSYFCDKCQKWIPASQLREQ
LSYLKGDNFFRFTCSDCSAD

Sequence Name: CRSP2BP
Full name:
UniGene Number: N.A.
Accession Number: NM_020536
Source organism: human ( Homo sapiens )
Full length size (aa): 782
NCBI information page: click here

Full length sequence:
MDSSIHLSSLISRHDDEATRTSTSEGLEEGEVEGETLLIVESEDQASVDL
SHDQSGDSLNSDEGDVSWMEEQLSYFCDKCQKWIPASQLREQLSYLKGDN
FFRFTCSDCSADGKEQYERLKLTWQQVVMLAMYNLSLEGSGRQGYFRWKE
DICAFIEKHWTFLLGNRKKTSTWWSTVAGCLSVGSPMYFRSGAQEFGEPG
WWKLVHNKPPTMKPEGEKLSASTLKIKAASKPTLDPIITVEGLRKRASRN
PVESAMELKEKRSRTQEAKDIRRAQKEAAGFLDRSTSSTPVKFISRGRRP
DVILEKGEVIDFSSLSSSDRTPLTSPSPSPSLDFSAPGTPASHSATPSLL
SEADLIPDVMPPQALFHDDDEMEGDGVIDPGMEYVPPPAGSVASGPVVGG
RKKVRGPEQIKQEVESEEEKPDRMDIDSEDTDSNTSLQTRAREKRKPQLE
KDTKPKEPRYTPVSIYEEKLLLKRLEACPGAVAMTPEARRLKRKLIVRQA
KRDRGLPLFDLDQVVNAALLLVDGIYGAKEGGISRLPAGQATYRTTCQDF
RILDRYQTSLPSRKGFRHQTTKFLYRLVGSEDMAVDQSIVSPYTSRILKP
YIRRDYETKPPKLQLLSQIRSHLHRSDPHWTPEPDAPLDYCYVRPNHIPT
INSMCQEFFWPGIDLSECLQYPDFSVVVLYKKVIIAFGFMVPDVKYNEAY
ISFLFVHPEWRRAGIATFMIYHLIQTCMGKDVTLHVSASNPAMLLYQKFG
FKTEEYVLDFYDKYYPLESTECKHAFFLRLRR