human ( Homo sapiens )
CAF40

Western result: +

GST GST-CAF40 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: CAF40
CBI Antibody Core Code: A0735
"Lot" Number: 1 Position: 100(199-298)
% Identical Homology of Target to Mouse Protein: 100% (NP_067358.1| rcd1 (required for cell differentiation) homolog 1; rcd1 (required for cell differentiation) homolog 1 (S.pombe))
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
ICQTYERFSHVAMILGKMVLQLSKEPSARLLKHVVRCYLR
LSDNPRAREALRQCLPDQLKDTTFAQVLKDDTTTKRWLAQ
LVKNLQEGQVTDPRGIPLPP

Sequence Name: CAF40
Full name: RCD1 required for cell differentiation1 homolog (S.pombe) (RQCD1)
UniGene Number: N.A.
Accession Number: NM_005444; RQCD1; NP_005435
Source organism: human ( Homo sapiens )
Full length size (aa): 299
NCBI information page: click here

Full length sequence:
MHSLATAAPVPTTLAQVDREKIYQWINELSSPETRENALLELSKKRESVP
DLAPMLWHSFGTIAALLQEIVNIYPSINPPTLTAHQSNRVCNALALLQCV
ASHPETRSAFLAAHIPLFLYPFLHTVSKTRPFEYLRLTSLGVIGALVKTD
EQEVINFLLTTEIIPLCLRIMESGSELSKTVATFILQKILLDDTGLAYIC
QTYERFSHVAMILGKMVLQLSKEPSARLLKHVVRCYLRLSDNPRAREALR
QCLPDQLKDTTFAQVLKDDTTTKRWLAQLVKNLQEGQVTDPRGIPLPPQ