human ( Homo sapiens )
NRCAM

Western result: +

control NRCAM-pep4 A0857 (fragment)

29.2 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: NRCAM-pep4
CBI Antibody Core Code: A0857
"Lot" Number: 1 Position: 20(561-580)
% Identical Homology of Target to Mouse Protein: 75% (NP_795904.2| neuronal cell adhesion molecule)
Molecular Weight of GST-fusion: 29.2 KDa

Target sequence used to make this antibody:
LWLKDNRELPSDERFTVDKD

Western result: +

control NRCAM-pep2 A0855 (fragment)

29.2 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: NRCAM-pep2
CBI Antibody Core Code: A0855
"Lot" Number: 1 Position: 20(239-258)
% Identical Homology of Target to Mouse Protein: 94% (NP_795904.2| neuronal cell adhesion molecule)
Molecular Weight of GST-fusion: 29.2 KDa

Target sequence used to make this antibody:
ISAKSSRERPPTFLTPEGNA

Sequence Name: NRCAM
Full name: Neuronal cell adhesion molecule precursor (Nr-CAM) (NgCAM-related cell adhesion molecule) (Ng-CAM-related) (hBravo)
UniGene Number: Hs.7912
Accession Number: NM_005010
Source organism: human ( Homo sapiens )
Full length size (aa): 1180
NCBI information page:

Sequence Notes: A0855, A0857: This antibody was generated from mice that were immunized with a mixed peptides from different regions of the antigen sequence. Serum was tested on individual peptide.

Full length sequence:
MQLKIMPKKKRLSAGRVPLILFLCQMISALEVPLDPKLLEDLVQPPTITQ
QSPKDYIIDPRENIVIQCEAKGKPPPSFSWTRNGTHFDIDKDPLVTMKPG
TGTLIINIMSEGKAETYEGVYQCTARNERGAAVSNNIVVRPSRSPLWTKE
KLEPITLQSGQSLVLPCRPPIGLPPPIIFWMDNSFQRLPQSERVSQGLNG
DLYFSNVLPEDTREDYICYARFNHTQTIQQKQPISVKVISAKSSRERPPT
FLTPEGNASNKEELRGNVLSLECIAEGLPTPIIYWAKEDGMLPKNRTVYK
NFEKTLQIIHVSEADSGNYQCIAKNALGAIHHTISVRVKAAPYWITAPQN
LVLSPGEDGTLICRANGNPKPRISWLTNGVPIEIAPDDPSRKIDGDTIIF
SNVQERSSAVYQCNASNEYGYLLANAFVNVLAEPPRILTPANTLYQVIAN
RPALLDCAFFGSPLPTIEWFKGAKGSALHEDIYVLHENGTLEIPVAQKDS
TGTYTCVARNKLGMAKNEVHLEIKDATWIVKQPEYAVVQRGSMVSFECKV
KHDHTLSLTVLWLKDNRELPSDERFTVDKDHLVVADVSDDDSGTYTCVAN
TTLDSVSASAVLSVVAPTPTPAPVYDVPNPPFDLELTDQLDKSVQLSWTP
GDDNNSPITKFIIEYEDAMHKPGLWHHQTEVSGTQTTAQLNLSPYVNYSF
RVMAVNSIGKSLPSEASEQYLTKASEPDKNPTAVEGLGSEPDNLVITWKP
LNGFESNGPGLQYKVSWRQKDGDDEWTSVVVANVSKYIVSGTPTFVPYLI
KVQALNDMGFAPEPAVVMGHSGEDLPMVAPGNVRVNVVNSTLAEVHWDPV
PLKSIRGHLQGYRIYYWKTQSSSKRNRRHIEKKILTFQGSKTHGMLPGLE
PFSHYTLNVRVVNGKGEGPASPDRVFNTPEGVPSAPSSLKIVNPTLDSLT
LEWDPPSHPNGILTEYTLKYQPINSTHELGPLVDLKIPANKTRWTLKNLN
FSTRYKFYFYAQTSAGSGSQITEEAVTTVDEAMASRQVDIATQGWFIGLM
CAVALLILILLIVCFIRRNKGGKYPVKEKEDAHADPEIQPMKEDDGTFGE
YSDAEDHKPLKKGSRTPSDRTVKKEDSDDSLVDYGEGVNGQFNEDGSFIG
QYSGKKEKEPAEGNESSEAPSPVNAMNSFV