human ( Homo sapiens )
p153

Western result: +

GST GST-p153 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: p153
CBI Antibody Core Code: A0937
"Lot" Number: 1 Position: 100 (132-231)
% Identical Homology of Target to Mouse Protein: 51% (NP_796268.2| RNA polymerase II associated protein 1)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
VFLRSRDTQGKSATSGKRSIFAQEIAARRIAEAKGPSVGE
VVPNVGPPEGAVTCETPTPRNQGCQLPGSSHSFQGPNLVT
GKGLRDQEAEQEAQTIHEEN

Sequence Name: p153
Full name: DKFZP727M111
UniGene Number: N.A.
Accession Number: NP_056355
Source organism: human ( Homo sapiens )
Full length size (aa): 1393
NCBI information page:

Full length sequence:
mlsrpkpgesevdllhfqsqflaagaapavqlvkkgnrgggdansdrppl
qdhrdvvmldnlpdlppalvpsppkrarpspghclpededpeerlrrhdq
hitavltkiierdtssvavnlpvpsgvafpavflrsrdtqgksatsgkrs
ifaqeiaarriaeakgpsvgevvpnvgppegavtcetptprnqgcqlpgs
shsfqgpnlvtgkglrdqeaeqeaqtiheeniarlqamapeeilqeqqrl
laqldpslvaflrshshtqeqtgetaseeqrpggpsanvtkeeplmsafa
seprkrdklepeapalalpvtpqkewlhmdtveleklhwtqdlppvrrqq
tqermqarfslqgellapdvdlpthlglhhhgeeaeragyslqelfhltr
sqvsqqralalhvlaqvisraqagefgdrlagsvlsllldagflfllrfs
lddrvdgviatairalrallvapgdeelldstfswyhgaltfplmpsqed
kedededeecpagkakrkspeeesrpppdlarhdvikgllatsllprlry
vlevtypgpavvldilavlirlarhslesatrvlecprlietivreflpt
swspvgagptpslykvpcatamkllrvlasagrniaarllssfdlrsrlc
riiaeapqelalppeeaemlstealrlwavaasygqggylyrelypvlmr
alqvvprelsthppqplsmqriaslltlltqltlaagstpaetisdsaea
slsatpslvtwtqvsglqplvepclrqtlkllsrpemwravgpvpvacll
flgayyqawsqqpsscpedwlqdmqrlseelllpllsqptlgslwdslrh
csllcnplscvpaleappslvslgcsggcprlslagsaspfpfltallsl
lntlaqihkglcgqlaailaapglqnyflqcvapgaaphltpfsawalrh
eyhlqylalalaqkaaalqplpathaalyhgmalallsrllpgseylthe
lllscvfrleflpertsggpeaadfsdqlslgssrvprcgqgtllaqacq
dlpsirncylthcsparasllasqalhrgelqrvptlllpmptepllptd
wpflplirlyhrasdtpsglsptdtmgtamrvlqwvlvleswrpqalwav
ppaarlarlmcvflvdselfrespvqhlvaallaqlcqpqvlpnlnldcr
lpgltsfpdlyanfldhfeavsfgdhlfgalvllplqrrfsvtlrlalfg
ehvgalralslpltqlpvslecytvppednlallqlyfrtlvtgalrprw
cpvlyavavahvnsfifsqdpqssdevkaarrsmlqktwlladeglrqhl
lhyklpnstlpegfelysqlpplrqhylqrltstvlqngvset