human ( Homo sapiens )
FIH

Western result: +/-

GST GST-FIH (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: FIH
CBI Antibody Core Code: A0168
"Lot" Number: 1 Position: 100(107-206)
% Identical Homology of Target to Mouse Protein: 97% (NP_795932.1| hypoxia-inducible factor 1, alpha subunit inhibitor)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
KMANFQNFKPRSNREEMKFHEFVEKLQDIQQRGGEERLYL
QQTLNDTVGRKIVMDFLGFNWNWINKQQGKRGWGQLTSNL
LLIGMEGNVTPAHYDEQQNF

Sequence Name: FIH
Full name: Factor inhibiting HIF1
UniGene Number: N.A.
Accession Number: AF395830
Source organism: human ( Homo sapiens )
Full length size (aa): 349
NCBI information page: click here

Full length sequence:
MAATAAEAVASGSGEPREEAGALGPAWDESQLRSYSFPTRPIPRLSQSDP
RAEELIENEEPVVLTDTNLVYPALKWDLEYLQENIGNGDFSVYSASTHKF
LYYDEKKMANFQNFKPRSNREEMKFHEFVEKLQDIQQRGGEERLYLQQTL
NDTVGRKIVMDFLGFNWNWINKQQGKRGWGQLTSNLLLIGMEGNVTPAHY
DEQQNFFAQIKGYKRCILFPPDQFECLYPYPVHHPCDRQSQVDFDNPDYE
RFPNFQNVVGYETVVGPGDVLYIPMYWWHHIESLLNGGITITVNFWYKGA
PTPKRIEYPLKAHQKVAIMRNIEKMLGEALGNPQEVGPLLNTMIKGRYN