tobacco hornworm ( Manduca sexta )
CRPR (Bt-R1-CYT)

Western result: +

GST GST-BtR1-LDV A0378 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: BtR1-LDV
CBI Antibody Core Code: A0378
"Lot" Number: 1 Position: 100(938-1037)
% Identical Homology of Target to Mouse Protein: 31% (NP_075859.1| cadherin 23; otocadherin; bustling; waltzer; modifier of deaf waddler; age related hearing loss 1; bobby)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
NRETGLVYVDYETQGSGEVLDRDGDEPTHRIFFNLIDNFM
GEGEGNRNQNDTEVLVILLDVNDNAPELPPPSELSWTISE
NLKQGVRLEPHIFAPDRDEP

Western result: +

GST GST-CRPR (Bt-R1-CYT) A0380 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: CRPR (Bt-R1-CYT)
CBI Antibody Core Code: A0380
"Lot" Number: 1 Position: 100(1611-1710)
% Identical Homology of Target to Mouse Protein: 36% (NP_038585.1| eukaryotic translation initiation factor 2 alpha kinase 1; heme-regulated inhibitor)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
TKYGSLDSGLNRAGIAAPGTNKHTVEGSNPIFNEAIKTPD
LDAISEGSNDSDLIGIEDLPHFGNVFMDPEVNEKANGYPE
VANHNNNFAFNPTPFSPEFV

Sequence Name: CRPR (Bt-R1-CYT)
Full name: CRPR (cadherin-related protein receptor); CRPR (Bt-R1-LDV)
UniGene Number: N.A.
Accession Number: AF319973
Source organism: tobacco hornworm ( Manduca sexta )
Full length size (aa): 1717
NCBI information page: click here

Full length sequence:
MAVDVRIAAFLLVFIAPAVLAQERCGYMTAIPRLPRPDNLPVLNFEGQTW
SQRPLLPAPERDDLCMDAYHVITANLGTQVIYMDEEIEDEITIAILNYNG
PSTPFIELPFLSGSYNLLMPVIRRVDNGEWHLIITQRQHYELPGMQQYMF
NVRVDGQSLVAGVSLAIVNIDDNAPIIQNFEPCRVPELGEPGLTECTYQV
SDADGRISTEFMTFRIDSVRGDEETFYIERTNIPNQWMWLNMTIGVNTSL
NFVTSPLHIFSVTALDSLPNTHTVTMMVQVANVNSRPPRWLEIFAVQQFE
EKSYQNFTVRAIDGDTEINMPINYRLITNEEDTFFSIEALPGGKSGAVFL
VSPIDRDTLQREVFPLTIVAYKYDEEAFSTSTNVVIIVTDINDQRPEPIH
KEYRLAIMEETPLTLNFDKEFGFHDKDLGQNAQYTVRLESVDPPGAAEAF
YIAPEVGYQRQTFIMGTLNHSMLDYEVPEFQSITIRVVATDNNDTRHVGV
ALVHIDLINWNDEQPIFEHAVQTVTFDETEGEGFFVAKAVAHDRDIGDVV
EHTLLGNAVNFLTIDKLTGDIRVSANDSFNYHRESELFVQVRATDTLGEP
FHTATSQLVIRLNDINNTPPTLRLPRGSPQVEENVPDGHVITQELRATDP
DTTADLRFEINWDTSFATKQGRQANPDEFRNCVEIETIFPEINNRGLAIG
RVVAREIRHNVTIDYEEFEVLSLTVRVRDLNTVYGDDYDESMLTITIIDM
NDNAPVWVEGTLEQNFRVREMSAGGLVVGSVRADDIDGPLYNQVRYTIFP
REDTDKDLIMIDFLTGQISVNTSGAIDADTPPRFHLYYTVVASDRCSTED
PADCPPDPTYWETEGNITIHITDTNNKVPQAETTKFDTVVYIYENATHLD
EVVTLIASDLDRDEIYHTVSYVINYAVNPRLMNFFSVNRETGLVYVDYET
QGSGEVLDRDGDEPTHRIFFNLIDNFMGEGEGNRNQNDTEVLVILLDVND
NAPELPPPSELSWTISENLKQGVRLEPHIFAPDRDEPDTDNSRVGYEILN
LSTERDIEVPELFVMIQIANVTGELETAMDLKGYWGTYAIHIRAFDHGIP
QMSMNETYELIIHPFNYYAPEFVFPTNDAVIRLARERAVINGVLATVNGE
FLERISATDPDGLHAGVVTFQVVGDEESQRYFQVVNDGENLGSLRLLQAV
PEEIREFRITIRATDQGTDPGPLSTDMTFRVVFVPTQGEPRFASSEHAVA
FIEKSAGMEESHQLPLAQDIKNHLCEDDCHSIYYRIIDGNSEGHFGLDPV
RNRLFLKKELIREQSASHTLQVAASNSPDGGIPLPASILTVTVTVREADP
RPVFVRELYTAGISTADSIGRELLRLHATQSEGSAITYAIDYDTMVVDPS
LEAVRQSAFVLNAQTGVLTLNIQPTATMHGLFKFEVTATDTAGAQDRTDV
TVYVVSSQNRVYFVFVNTLQQVEDNRDFIADTFSAGFNMTCNIDQVVPAN
DPVTGVALEHSTQMRGHFIRDNVPVLADEIEQIRSDLVLLSSIQTTLAAR
SLVLQDLLTNSSPDSAPDSSLTVYVLASLSAVLGFMCLVLLLTFIIRTRA
LNRRLEALSMTKYGSLDSGLNRAGIAAPGTNKHTVEGSNPIFNEAIKTPD
LDAISEGSNDSDLIGIEDLPHFGNVFMDPEVNEKANGYPEVANHNNNFAF
NPTPFSPEFVNGQFRKI