malaria parasite (malaria (P. falciparum)) ( Plasmodium falciparum )
PFE0415w

Western result: +

GST GST-pfTFIIB-Like (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: pfTFIIB-Like
CBI Antibody Core Code: A1233
"Lot" Number: 1 Position: 100 (195-294)
% Identical Homology of Target to Mouse Protein: 32% (XP_485015.1| RIKEN cDNA C630007C17 gene)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
TTQKKTTIKNVIKTKNLKREDMETENEQNEAQPNNEKNEA
QPNNEKNKAPPNDEKNEAPSNEENVVQDLNKDELSDNLEN
NPVKNFDTDFENKNDGEEKY

Sequence Name: PFE0415w
Full name: transcription factor iib, putative
UniGene Number: N.A.
Accession Number: NP_703429
Source organism: malaria parasite (malaria (P. falciparum)) ( Plasmodium falciparum )
Full length size (aa): 360
NCBI information page: click here

Full length sequence:
MYRNKYQPNILSLLLSAGSKPLEIWKTKTKKGCVRKVLDDTIKLSAIEIL
SENTSDSYIYTAPQPFKSLSITLPIIMNKYFSFRISILDDKRCRRTFRIS
NFQTVTRLSNKWCTMPMVLNEGWNIIQINLNDYTEKAFRTKYKETIDIQI
NASIRIRCVYFCDRIYKNEELKDEFKIFCKKKEKPKYTPPQYLNTTQKKT
TIKNVIKTKNLKREDMETENEQNEAQPNNEKNEAQPNNEKNKAPPNDEKN
EAPSNEENVVQDLNKDELSDNLENNPVKNFDTDFENKNDGEEKYLIDVID
NAEEDGEDKILENQKEENENENPYNEVDITNISEIKNLEMDLNNVLYEDV
NYETYNNDDN