Norway rat (Rat) ( Rattus norvegicus )
Atcay

Western result: +

GST GST-Atcay (fragment)

19.83 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Atcay
CBI Antibody Core Code: A1240
"Lot" Number: 1 Position: 53 (320-372)
% Identical Homology of Target to Mouse Protein: 96% (NP_848777.1| ataxia, cerebellar, Cayman type homolog; hesitant; jittery)
Molecular Weight of fusion protein: 19.83 KDa

Target sequence used to make this antibody:
VLQYEEQRLRAKRESARPPQPEFLLPRSEEKPETVEEEDR
AAEVTEDQETSMS

Sequence Name: Atcay
Full name:
UniGene Number: N.A.
Accession Number: N.A.
Source organism: Norway rat (Rat) ( Rattus norvegicus )
Full length size (aa): 372
NCBI information page:

Full length sequence:
MGTTEATLRMENVDVRDEWQDEDLPRPLPEDTGEDHLGGTVEDSSSPPST
LNLSGAHRKRKTLVAPEINISLDQSEGSLLSDDFLDTPDDLDINVDDIET
PDETDSLEFLGNGNELEWEDDTPVATAKNMPGDSADLFGDGSGEDGSAAN
GRLWRTVIIGEQEHRIDLHMIRPYMKVVTHGGYYGEGLNAIIVFAACFLP
DSSSPDYHYIMENLFLYVISSLELLVAEDYMIVYLNGATPRRRMPGIGWL
KKCYHMIDRRLRKNLKSLIIVHPSWFIRTVLAISRPFISVKFISKIQYVH
SLEELEQLIPMEHVQLPACVLQYEEQRLRAKRESARPPQPEFLLPRSEEK
PETVEEEDRAAEVTEDQETSMS