Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Rad14

Western result: +

control yRad14 A1066b (fragment)

32.2 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: yRad14
CBI Antibody Core Code: A1066b
"Lot" Number: 1 Position: 20 (288-307)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of GST-fusion: 32.2 KDa

Target sequence used to make this antibody:
DEEWQRREEGKAHRREKKYE

Antibody Name/Abbreviation: yRad14
CBI Antibody Core Code: A1066a
"Lot" Number: 1 Position: 100 (88-187)
% Identical Homology of Target to Mouse Protein: 33% (XP_485461.1| PREDICTED: ubiquitin specific protease 31)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
GVVDGSKRDASVLDKRPTDRIRPSIRKQDYIEYDFATMQN
LNGGYINPKDKLPNSDFTDDQEFESEFGSKKQKTLQDWKK
EQLERKMLYENAPPPEHISK

Sequence Name: Rad14
Full name:
UniGene Number: N.A.
Accession Number: YMR201C
Source organism: Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Full length size (aa): 371
NCBI information page:

Full length sequence:
MTPEQKAKLEANRKLAIERLRKRGILSSDQLNRIESRNEPLKTRPLAVTS
GSNRDDNAAAAVHVPNHNGQPSALANTNTNTTSLYGSGVVDGSKRDASVL
DKRPTDRIRPSIRKQDYIEYDFATMQNLNGGYINPKDKLPNSDFTDDQEF
ESEFGSKKQKTLQDWKKEQLERKMLYENAPPPEHISKAPKCIECHINIEM
DPVLHDVFKLQVCKQCSKEHPEKYALLTKTECKEDYFLTDPELNDEDLFH
RLEKPNPHSGTFARMQLFVRCEVEAFAFKKWGGEEGLDEEWQRREEGKAH
RREKKYEKKIKEMRLKTRAQEYTNRLREKKHGKAHIHHFSDPVDGGIDED
GYQIQRRRCTDCGLETEEIDI