Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
FCP1

Western result: +

GST GST-FCP1 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: FCP1
CBI Antibody Core Code: A0619
"Lot" Number: 2 Position: 100(1-100)
% Identical Homology of Target to Mouse Protein: 35% (XP_145358.1| similar to zinc finger protein 494)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
MTTQIRSPQGLPYPIQIDKLIPSVGSYLHEGDRLLVYKFW
YLVERASDTGDDDNEHDVSPGGSAGSNGVSPPTKQLRESI
EFFESPYEGDLISWNVDVGD

Sequence Name: FCP1
Full name: YMR277W
UniGene Number: N.A.
Accession Number: NC_001145.1
Source organism: Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Full length size (aa): 732
NCBI information page: click here

Full length sequence:
MTTQIRSPQGLPYPIQIDKLIPSVGSYLHEGDRLLVYKFWYLVERASDTG
DDDNEHDVSPGGSAGSNGVSPPTKQLRESIEFFESPYEGDLISWNVDVGD
EVATANQVICEIKRPCNHDIVYGGLCTQCGKEVSADAFDGVPLDVVGDVD
LQISETEAIRTGKALKEHLRRDKKLILVVDLDQTIIHCGVDPTIAEWKND
PNNPNFETLRDVKSFTLDEELVLPLMYMNDDGSMLRPPPVRKCWYYVKVR
PGLKEFFAKVAPLFEMHIYTMATRAYALQIAKIVDPTGELFGDRILSRDE
NGSLTTKSLAKLFPTDQSMVVVIDDRGDVWNWCPNLIKVVPYNFFVGVGD
INSNFLPKQSTGMLQLGRKTRQKSQESQELLTDIMDNEKKLQEKIDKEVK
RQEEKLNHQLATAEEPPANESKEELTKKLEYSASLEVQQQNRPLAKLQKH
LHDQKLLVDDDDELYYLMGTLSNIHKTYYDMLSQQNEPEPNLMEIIPSLK
QKVFQNCYFVFSGLIPLGTDIQRSDIVIWTSTFGATSTPDIDYLTTHLIT
KNPSTYKARLAKKFNPQIKIVHPDWIFECLVNWKKVDEKPYTLIVDSPIS
DEELQNFQTQLQKRQEYLEETQEQQHMLTSQENLNLFAAGTSWLNNDDDE
DIPDTASDDDEDDDHDDESDDENNSEGIDRKRSIEDNHDDTSQKKTKAEP
SQDGPVQHKGEGDDNEDSDSQLEEELMDMLDD