Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Ndd1p

Western result: +

GST GST-Ndd1p (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Ndd1p
CBI Antibody Core Code: A0779
"Lot" Number: 2 Position: 100(165-264)
% Identical Homology of Target to Mouse Protein: 36% (NP_038599.1| kinase suppressor of ras)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
LLDSPSTNFNFFHKTPAKTPLRFVTDSNGAQQSTTENPGQ
QQNVFSNVDLNNLLKSNGKTPSSSCTGAFSRTPLSKIDMN
LMFNQPLPTSPSKRFSSLSL

Sequence Name: Ndd1p
Full name: ORF YOR372c
UniGene Number: N.A.
Accession Number: Z75280 (Y13140)
Source organism: Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Full length size (aa): 554
NCBI information page: click here

Full length sequence:
MDRDISYQQNYTSTGATATSSRQPSTDNNADTNFLKVMSEFKYNFNSPLP
TTTQFPTPYSSNQYQQTQDHFANTDAHNSSSNESSLVENSILPHHQQIQQ
QQQQQQQQQQQQQALGSLVPPAVTRTDTSETLDDINVQPSSVLQFGNSLP
SEFLVASPEQFKEFLLDSPSTNFNFFHKTPAKTPLRFVTDSNGAQQSTTE
NPGQQQNVFSNVDLNNLLKSNGKTPSSSCTGAFSRTPLSKIDMNLMFNQP
LPTSPSKRFSSLSLTPYGRKILNDVGTPYAKALISSNSALVDFQKARKDI
TTNATSIGLENANNILQRTPLRSNNKKLFIKTPQDTINSTSTLTKDNENK
QDIYGSSPTTIQLNSSITKSISKLDNSRIPLLASRSDNILDSNVDDQLFD
LGLTRLPLSPTPNCNSLHSTTTGTSALQIPELPKMGSFRSDTGINPISSS
NTVSFKSKSGNNNSKGRIKKNGKKPSKFQIIVANIDQFNQDTSSSSLSSS
LNASSSAGNSNSNVTKKRASKLKRSQSLLSDSGSKSQARKSCNSKSNGNL
FNSQ