Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
yRAD16

Western result: +

GST GST-yRAD16 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: yRAD16
CBI Antibody Core Code: A0309
"Lot" Number: 3 Position: 100(48-147)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
DSQDEEYRDDATLVKSPDDDDKDFIIDLTGSDKERTATDE
NTHAIKNDNDEIIEIKEERDVSDDDEPLTKKRKTTARKKK
KKTSTKKKSPKVTPYERNTL

Sequence Name: yRAD16
Full name: ORF YBR114w
UniGene Number: N.A.
Accession Number: CAA85071
Source organism: Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Full length size (aa): 790
NCBI information page: click here

Full length sequence:
MQEGGFIRRRRTRSTKKSVNYNELSDDDTAVKNSKTLQLKGNSENVNDSQ
DEEYRDDATLVKSPDDDDKDFIIDLTGSDKERTATDENTHAIKNDNDEII
EIKEERDVSDDDEPLTKKRKTTARKKKKKTSTKKKSPKVTPYERNTLRLY
EHHPELRNVFTDLKNAPPYVPQRSKQPDGMTIKLLPFQLEGLHWLISQEE
SIYAGGVLADEMGMGKTIQTIALLMNDLTKSPSLVVAPTVALMQWKNEIE
QHTKGQLKIYIYHGASRTTDIKDLQGYDVVLTTYAVLESVFRKQNYGFRR
KNGLFKQPSVLHNIDFYRVILDEAHNIKDRQSNTARAVNNLKTQKRWCLS
GTPLQNRIGEMYSLIRFLNINPFTKYFCTKCDCASKDWKFTDRMHCDHCS
HVIMQHTNFFNHFMLKNIQKFGVEGPGLESFNNIQTLLKNIMLRRTKVER
ADDLGLPPRIVTVRRDFFNEEEKDLYRSLYTDSKRKYNSFVEEGVVLNNY
ANIFTLITRMRQLADHPDLVLKRLNNFPGDDIGVVICQLCNDEAEEPIES
KCHHKFCRLCIKEYVESFMENNNKLTCPVCHIGLSIDLSQPALEVDLDSF
KKQSIVSRLNMSGKWQSSTKIEALVEELYKLRSNKRTIKSIVFSQFTSML
DLVEWRLKRAGFQTVKLQGSMSPTQRDETIKYFMNNIQCEVFLVSLKAGG
VALNLCEASQVFILDPWWNPSVEWQSGDRVHRIGQYRPVKITRFCIEDSI
EARIIELQEKKANMIHATINQDEAAISRLTPADLQFLFNN