Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
TAF9 (TAF17)

Western result: +

GST GST-TAF9 (TAF17) (fragment)

30.63 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: TAF9 (TAF17)
CBI Antibody Core Code: A0969
"Lot" Number: 1 Position: 33 (1-33)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of fusion protein: 30.63 KDa

Target sequence used to make this antibody:
MNGGGKNVLNKNSVGSVSEVGPDSTQEETPRDV

Sequence Name: TAF9 (TAF17)
Full name: YMR236W
UniGene Number: N.A.
Accession Number: N.A.
Source organism: Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Full length size (aa): 157
NCBI information page:

Full length sequence:
MNGGGKNVLNKNSVGSVSEVGPDSTQEETPRDVRLLHLLLASQSIHQYED
QVPLQLMDFAHRYTQGVLKDALVYNDYAGSGNSAGSGLGVEDIRLAIAAR
TQYQFKPTAPKELMLQLAAERNKKALPQVMGTWGVRLPPEKYCLTAKEWD
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