Salmonella ( Salmonella typhimurium )
SopE2

Western result: +

GST GST-SopE2 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: SopE2
CBI Antibody Core Code: A0192
"Lot" Number: 2 Position: 100(40-139)
% Identical Homology of Target to Mouse Protein: 33% (XP_489633.1| similar to armadillo repeat-containing protein)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
NSFISLSTSLSDRFSLHQQTDIPTTHFHRGNASEGRAVLT
SKTVKDFMLQKLNSLDIKGNASKDPAYARQTCEAILSAVY
SNNKDQCCKLLISKGVSITP

Other western:
Code: A0192
Lot: 1

Western result: +

GST GST-SopE2 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: SopE2
Full name:
UniGene Number: N.A.
Accession Number: AAL20770
Source organism: Salmonella ( Salmonella typhimurium )
Full length size (aa): 240
NCBI information page:

Full length sequence:
MTNITLSTQHYRIHRSDVEPVKEKTTEKDIFAKSITAVRNSFISLSTSLS
DRFSLHQQTDIPTTHFHRGNASEGRAVLTSKTVKDFMLQKLNSLDIKGNA
SKDPAYARQTCEAILSAVYSNNKDQCCKLLISKGVSITPFLKEIGEAAQN
AGLPGEIKNGVFTPGGAGANPFVVPLIASASIKYPHMFINHNQQVSFKAY
AEKIVMKEVTPLFNKGTMPTPQQFQLTIENIANKYLQNAS