fission yeast ( Schizosaccharomyces pombe )
INO80

Western result: +

GST GST-INO80 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: INO80
CBI Antibody Core Code: A0184
"Lot" Number: 1 Position: 100(1-100)
% Identical Homology of Target to Mouse Protein: 36% (XP_489569.1| similar to KIAA2018 protein)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
MDPSRETFDGSTRDSYKPPNGAEMMGQSELNTQNRIPYNT
SANNRNWQIPLYWQQRGNEFQASPPPPLGYVTPEYGATGT
PVNANNASRVDYATTAANVP

Sequence Name: INO80
Full name:
UniGene Number: N.A.
Accession Number: N.A.
Source organism: fission yeast ( Schizosaccharomyces pombe )
Full length size (aa): 1604
NCBI information page:

Full length sequence:
MDPSRETFDGSTRDSYKPPNGAEMMGQSELNTQNRIPYNTSANNRNWQIP
LYWQQRGNEFQASPPPPLGYVTPEYGATGTPVNANNASRVDYATTAANVP
EEYANDYSSELAYIHNVNDMPHVDGLSNHSPATQPDLFETPAQSDPILFS
SYPHAAQARVDPSISKDLYNMVPRPDANTVSPHAARSASSLPVPKEASET
PFRDASTDLFDEHAHAAPMHSSISISTLLSDSDRYEPHVSLTENISPVMA
PSIDARLSQTILRGLPPAQKLSPNSSQSQITHNRRKHKLPLNATTNNSVV
LTPDTSPLLDSDEVVSDDDSNEQQTMMMKFNYLQHLRNKRDEAVHAEKRR
LLDIRGSIHDRLVCRYENRYNKLHASEYNHHHDWAVRQAIREEVAAVEAA
KIRADEEKKKKEREEQVRLLQESADKDAEMNEASTATSENEDLKDDLSLA
DLSSKKTANSQATENNNTPSKAKVKAESKVRSKAKSDKSRAKLSSDTNKD
SEKNDNNDASLQSAGVASDGESSPETPLTKASKSKKAKASKLANDTSKNA
NGETKSTPKKSKKKTSKAQQEANSTTAEGKEKLSGDSTETGNSTNKEAST
EDTKANATASAPNKKKKTVETLQQQVIKEIARKEIPRVYKIIQQNQYNRS
TNARKTSQLCGREARRWQFRTIKNNKDMQTKAKRAMRETMVFWKRNERVE
RDLRKKAEREALDRAKKEEELRESRRQARKLDFLITQTELYSHFVGRKMD
REQDLPSATNTASVSEINFDSDEEEDIRRLAVESAQEAVQKAREHSQLFD
ANRQQSPNNSSSDMNEGEMNFQNPTLVNAFEVKQPKMLMCKLKEYQLKGL
NWLANLYEQGINGILADEMGLGKTVQSISVMAYLAETHNIWGPFLVIAPA
STLHNWQQEITRFVPKLKCIPYWGSTKDRKILRKFWCRKNMTYDENSPFH
VVVTSYQLVVLDAQYFQSVKWQYMILDEAQAIKSSSSSRWKSLLAFKCRN
RLLLTGTPIQNTMQELWALLHFIMPSLFDSHNEFSEWFSKDIESHAQSNT
QLNEQQLKRLHMILKPFMLRRVKKNVQSELGEKIEKEVYCDLTQRQKILY
QALRRQISIAELLEKAILGGDDTVASIMNLVMQFRKVCNHPDLFEREDVR
SPLSLATWSKSIYINREGNFLDVPYNTRNFITFSIPRLLYEQGGILSVPG
LNTSRGFETKYLYNLMNIWNPEYTNDSIKSNPEGSPFSWLRFVDESPQTL
FQTFQNPVVHYLDEAEASSSLKEEQLCRQEFCYGKDYSNVRKMLLLPKSI
TKVDVLGSDFKEDSPFYHLTHVLEESDSQLDLTLLDSVLVQRASAPPIDI
YCPGSRQFTVLQSRFQRDHLWSHYLYQPLKGEEDLIINNQAVSKLPIPRK
PLLPSFGIAKGSYSNVRIPSMLRFIADSGKLSKLDKLLVELKANDHRVLI
YFQMTRMIDLMEEYLTFRQYKYLRLDGSSKISQRRDMVTEWQTRPELFVF
LLSTRAGGLGINLTAADTVIFYDSDWNPSIDSQAMDRAHRIGQQKQVTVY
RFITRGTIEERIVIRAKEKEEVQKVVISGGETRPTKQMDLKGNSREMVSW
LLEE